Zahedan Journal of Research in Medical Sciences

Published by: Kowsar

Evolution of Antimicrobial Activity of Leaf Extract of Withania somnifera Against Antibiotic Resistanct Staphylococcus aureus

Mohammad Bokaeian 1 and Saeide Saeidi 2 , *
Authors Information
1 Department of Laboratory Sciences, Faculty of Paramedical Sciences, Research Center for Infectious Diseases and Tropical Medicine, Zahedan University of Medical Sciences, Zahedan, IR Iran
2 Department of Microbiology, Kerman Sciences and Researches Branch, Islamic Azad University, Kerman, IR Iran
Article information
  • Zahedan Journal of Research in Medical Sciences: July 28, 2015, 17 (7)
  • Published Online: July 25, 2015
  • Article Type: Research Article
  • Received: January 2, 2014
  • Accepted: March 28, 2014
  • DOI: 10.17795/zjrms1016

To Cite: Bokaeian M, Saeidi S. Evolution of Antimicrobial Activity of Leaf Extract of Withania somnifera Against Antibiotic Resistanct Staphylococcus aureus, Zahedan J Res Med Sci. 2015 ; 17(7):-. doi: 10.17795/zjrms1016.

Copyright © 2015, Zahedan University of Medical Sciences.This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Patients and Methods
4. Results
5. Discussion
  • 1. Swarnkar S, Katewa SS. Antimicromrobial activities of some tuberous medicinal plants from aravalli hills of Rajasthan. J Herbal Med Toxicol. 2009; 3(1): 53-8
  • 2. Thambiraj J, Paulsamy S. Antimicrobial screening of stem extract of the folklore medicinal plant, Acalypha fruticosa Forssk. Int J Pharm Pharm Sci. 2011; 3(4): 285-7
  • 3. De Britto J, Gracelin HS. Datura metel–a plant with potential as antibacterial agent. Int J Appl Biol Pharm Technol. 2011; 2(2): 429-33
  • 4. Chopra RN, Dasgupta NK. A handbook of applied pharmacology and therapeutics: Including materia medica. 1963; : 331
  • 5. Mehrotra V, Mehrotra S, Kirar V, Shyam R, Misra K, Srivastava AK, et al. Antioxidant and antimicrobial activities of aqueous extract of Withania somnifera against methicillin-resistant Staphylococcus aureus. J Microbiol Biotechnol Res. 2011; 1(1): 40-5
  • 6. Afolayan AJ, Grierson DS, Kambizi L, Madamombe I, Masika PJ, Jäger AK. In vitro antifungal activity of some South Afr medicinal plants. South Afr J Botany. 2002; 68(1): 72-6[DOI]
  • 7. Arora S, Dhillon S, Rani G, Nagpal A. The in vitro antibacterial/synergistic activities of Withania somnifera extracts. Fitoterapia. 2004; 75(3-4): 385-8[DOI][PubMed]
  • 8. Jain P, Varshney R. Antimicrobial activity of aqueous and methanolic extracts of Withania somnifera (Ashwagandha). J Chem Pharm Res. 2011; 3(3): 260-3
  • 9. Emboden WA. Narcotic plants. 1997;
  • 10. Payne MC, Wood HF, Karakawa W, Gluck L. A prospective study of staphylococcal colonization and infections in newborns and their families. Am J Epidemiol. 1965; 82(3): 305-16[PubMed]
  • 11. Chihara S, Popovich KJ, Weinstein RA, Hota B. Staphylococcus aureus bacteriuria as a prognosticator for outcome of Staphylococcus aureus bacteremia: a case-control study. BMC Infect Dis. 2010; 10: 225[DOI][PubMed]
  • 12. Well B, Stood RJ. Bergy's manual of systemic bacteriology. 2012; : 423-427
  • 13. Forbes BA, Sahm. D. F. , Weissfeld AS. Bailey & Scott`s diagnostic microbiology. 2007;
  • 14. Clinical and Laboratory Standards Institute. Performance standards for antimicrobial disk susceptibility testing
  • 15. Duran N, Ozer B, Duran GG, Onlen Y, Demir C. Antibiotic resistance genes & susceptibility patterns in staphylococci. Indian J Med Res. 2012; 135: 389-96[PubMed]
  • 16. Zarifian A, Sadeghian A, Sadeghian H, Ghazvini K, Safdari H. Antibiotic resistance pattern of hospital isolates of Staphylococcus aureus in Mashhad-Iran during 2009 - 2011. Arch Clin Infect Dis. 2012; 7(3): 96-8[DOI]
  • 17. Khalili H, Soltani R, Gholami K, Rasoolinejad M, Abdollahi A. Antimicrobial susceptibility pattern of Staphylococcus aureus strains isolated from hospitalized patients in Tehran, Iran. Iran J Pharm Sci. 2010; 6(2): 125-32
  • 18. Ghasemian R, Najafi N, Makhlough A, Khademloo M. Frequency of nasal carriage of Staphylococcus aureus and its antimicrobial resistance pattern in patients on hemodialysis. Iran J Kidney Dis. 2010; 4(3): 218-22[PubMed]
  • 19. Rahimi F, Bouzari M, Maleki Z, Rahimi F. Antibiotic susceptibility pattern among Staphylococcus spp. with emphasis on detection of mecA gene in methicillin resistant Staphylococcus aureus isolates. Arch Clin Infect Dis. 2009; 4(3): 143-50
  • 20. Velu S, Baskaran C. Phytochemical analysis and in-vitro antimicrobial activity of Withania somnifera (Ashwagandha). J Nat Prod Plant Resour. 2012; 2(6): 711
  • 21. Srinu B, Kumar BV, Rao LV, Kalakumar B, Rao TM, Reddy AG. Screening of antimicrobial activity of Withania somnifera and Aloe vera plant extracts against food borne pathogens. J Chem Pharm Res. 2012; 4(11): 4800
  • 22. Pandit S, Chang KW, Jeon JG. Effects of Withania somnifera on the growth and virulence properties of Streptococcus mutans and Streptococcus sobrinus at sub-MIC levels. Anaerobe. 2013; 19: 1-8[DOI][PubMed]
  • 23. Alam N, Hossain M, Mottalib MA, Sulaiman SA, Gan SH, Khalil MI. Methanolic extracts of Withania somnifera leaves, fruits and roots possess antioxidant properties and antibacterial activities. BMC Complement Altern Med. 2012; 12: 175[DOI][PubMed]
  • 24. Datta S, Kumar Pal NK, Nandy AK. Inhibition of the emergence of multi drug resistant Staphylococcus aureus by Withania somnifera root extracts. Asian Pac J Trop Med. 2011; 4(11): 917-20[DOI][PubMed]
  • 25. Lee HS, Kim MJ. Selective responses of three Ginkgo biloba leaf-derived constituents on human intestinal bacteria. J Agric Food Chem. 2002; 50(7): 1840-4[PubMed]
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