Zahedan Journal of Research in Medical Sciences

Published by: Kowsar

Inhibitory Effect of Biologically Synthesized Silver Nanoparticle on Growth and Virulence of E. coli

Abass Tanhaeian 1 and Farajollah Shahriari Ahmadi 1 , *
Authors Information
1 Department of Biotechnology and Plant Breeding, College of Agriculture, Ferdowsi University of Mashhad (FUM), Mashhad, Iran
Article information
  • Zahedan Journal of Research in Medical Sciences: June 2018, 20 (6); e10269
  • Published Online: September 11, 2018
  • Article Type: Research Article
  • Received: December 31, 2016
  • Revised: January 24, 2018
  • Accepted: March 5, 2018
  • DOI: 10.5812/zjrms.10269

To Cite: Tanhaeian A, Shahriari Ahmadi F. Inhibitory Effect of Biologically Synthesized Silver Nanoparticle on Growth and Virulence of E. coli, Zahedan J Res Med Sci. 2018 ; 20(6):e10269. doi: 10.5812/zjrms.10269.

Copyright © 2018, Zahedan Journal of Research in Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Methods
4. Results
5. Discussion
  • 1. Nowack B, Krug HF, Height M. 120 years of nanosilver history: Implications for policy makers. Environ Sci Technol. 2011;45(4):1177-83. doi: 10.1021/es103316q. [PubMed: 21218770].
  • 2. Kannan N, Subbalaxmi S. Biogenesis of nanoparticles - A current perspective. Rev Adv Mater Sci. 2011;27(2):99-114.
  • 3. Mandal D, Bolander ME, Mukhopadhyay D, Sarkar G, Mukherjee P. The use of microorganisms for the formation of metal nanoparticles and their application. Appl Microbiol Biotechnol. 2006;69(5):485-92. doi: 10.1007/s00253-005-0179-3. [PubMed: 16317546].
  • 4. Cui H, Feng Y, Ren W, Zeng T, Lv H, Pan Y. Strategies of large scale synthesis of monodisperse nanoparticles. Recent Pat Nanotechnol. 2009;3(1):32-41. doi: 10.2174/187221009787003302. [PubMed: 19149753].
  • 5. Suresh AK, Pelletier DA, Wang W, Moon JW, Gu B, Mortensen NP, et al. Silver nanocrystallites: Biofabrication using Shewanella oneidensis, and an evaluation of their comparative toxicity on Gram-negative and Gram-positive bacteria. Environ Sci Technol. 2010;44(13):5210-5. doi: 10.1021/es903684r. [PubMed: 20509652].
  • 6. Sedighi I, Nejad AS, Amanati A, Nakhaei S, Alikhani MY. Virulence factors and antibiotic resistance in uropathogenic and commensal Escherichia coli isolates. J Krishna Inst Med Sci Univ. 2016;5(4):50-7.
  • 7. Haghighatpanah M, Mozaffari Nejad AS, Mojtahedi A, Amirmozafari N, Zeighami H. Detection of extended-spectrum beta-lactamase (ESBL) and plasmid-borne blaCTX-M and blaTEM genes among clinical strains of Escherichia coli isolated from patients in the north of Iran. J Glob Antimicrob Resist. 2016;7:110-3. doi: 10.1016/j.jgar.2016.08.005. [PubMed: 27721192].
  • 8. Bielaszewska M, Ruter C, Kunsmann L, Greune L, Bauwens A, Zhang W, et al. Enterohemorrhagic Escherichia coli hemolysin employs outer membrane vesicles to target mitochondria and cause endothelial and epithelial apoptosis. PLoS Pathog. 2013;9(12). e1003797. doi: 10.1371/journal.ppat.1003797. [PubMed: 24348251]. [PubMed Central: PMC3861543].
  • 9. Skals M, Jorgensen NR, Leipziger J, Praetorius HA. Alpha-hemolysin from Escherichia coli uses endogenous amplification through P2X receptor activation to induce hemolysis. Proc Natl Acad Sci U S A. 2009;106(10):4030-5. doi: 10.1073/pnas.0807044106. [PubMed: 19225107]. [PubMed Central: PMC2656199].
  • 10. Mahdieh M, Zolanvari A, Azimee AS, Mahdieh M. Green biosynthesis of silver nanoparticles by Spirulina platensis. Scientia Iranica. 2012;19(3):926-9. doi: 10.1016/j.scient.2012.01.010.
  • 11. Yasin S, Liu L, Yao J. Biosynthesis of Silver Nanoparticles by Bamboo Leaves Extract and Their Antimicrobial Activity. J Fiber Bioengineer Informatic. 2013:77-84. doi: 10.3993/jfbi03201307.
  • 12. Xiang H, Qiu JZ, Wang DC, Jiang YS, Xia LJ, Deng XM. Influence of magnolol on the secretion of alpha-toxin by Staphylococcus aureus. Molecules. 2010;15(3):1679-89. doi: 10.3390/molecules15031679. [PubMed: 20336007].
  • 13. Mehta SK, Gaur JP. Use of algae for removing heavy metal ions from wastewater: Progress and prospects. Crit Rev Biotechnol. 2005;25(3):113-52. doi: 10.1080/07388550500248571. [PubMed: 16294830].
  • 14. Jyoti K, Baunthiyal M, Singh A. Characterization of silver nanoparticles synthesized using Urtica dioica Linn. leaves and their synergistic effects with antibiotics. J Radiation Res Appl Sci. 2016;9(3):217-27. doi: 10.1016/j.jrras.2015.10.002.
  • 15. Korbekandi H, Asghari G, Chitsazi MR, Bahri Najafi R, Badii A, Iravani S. Green biosynthesis of silver nanoparticles using Althaea officinalis radix hydroalcoholic extract. Artif Cells Nanomed Biotechnol. 2016;44(1):209-15. doi: 10.3109/21691401.2014.936064. [PubMed: 25058031].
  • 16. Dhand V, Soumya L, Bharadwaj S, Chakra S, Bhatt D, Sreedhar B. Green synthesis of silver nanoparticles using Coffea arabica seed extract and its antibacterial activity. Mater Sci Eng C Mater Biol Appl. 2016;58:36-43. doi: 10.1016/j.msec.2015.08.018. [PubMed: 26478284].
  • 17. DhanaLakshmi D, Riyazulla A, Rekha R, Poonkodi S, Thangaraju N. Synthesis of silver nanoparticles using green and brown seaweeds. Phykos. 2012;42(2):39-45.
  • 18. Prasad TNVKV, Kambala VSR, Naidu R. Phyconanotechnology: synthesis of silver nanoparticles using brown marine algae Cystophora moniliformis and their characterisation. J Appl Phycol. 2012;25(1):177-82. doi: 10.1007/s10811-012-9851-z.
  • 19. Rai M, Yadav A, Gade A. Silver nanoparticles as a new generation of antimicrobials. Biotechnol Adv. 2009;27(1):76-83. doi: 10.1016/j.biotechadv.2008.09.002. [PubMed: 18854209].
  • 20. MubarakAli D, Thajuddin N, Jeganathan K, Gunasekaran M. Plant extract mediated synthesis of silver and gold nanoparticles and its antibacterial activity against clinically isolated pathogens. Colloids Surf B Biointerfaces. 2011;85(2):360-5. doi: 10.1016/j.colsurfb.2011.03.009. [PubMed: 21466948].
  • 21. Morones JR, Elechiguerra JL, Camacho A, Holt K, Kouri JB, Ramirez JT, et al. The bactericidal effect of silver nanoparticles. Nanotechnology. 2005;16(10):2346-53. doi: 10.1088/0957-4484/16/10/059. [PubMed: 20818017].
  • 22. Sondi I, Salopek-Sondi B. Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria. J Colloid Interface Sci. 2004;275(1):177-82. doi: 10.1016/j.jcis.2004.02.012. [PubMed: 15158396].
  • 23. Das B, Dash SK, Mandal D, Adhikary J, Chattopadhyay S, Tripathy S, et al. Green-synthesized silver nanoparticles kill virulent multidrug-resistant Pseudomonas aeruginosa strains: A mechanistic study. BLDE Univ J Health Sci. 2016;1(2):89. doi: 10.4103/2468-838X.196087.
  • 24. Bubeck Wardenburg J, Bae T, Otto M, Deleo FR, Schneewind O. Poring over pores: alpha-hemolysin and Panton-Valentine leukocidin in Staphylococcus aureus pneumonia. Nat Med. 2007;13(12):1405-6. doi: 10.1038/nm1207-1405. [PubMed: 18064027].
  • 25. Ragle BE, Bubeck Wardenburg J. Anti-alpha-hemolysin monoclonal antibodies mediate protection against Staphylococcus aureus pneumonia. Infect Immun. 2009;77(7):2712-8. doi: 10.1128/IAI.00115-09. [PubMed: 19380475]. [PubMed Central: PMC2708543].
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:



Create Citiation Alert
via Google Reader

Readers' Comments