Effects of Curcuma longa Extract on Telomerase Activity in Lung and Breast Cancer Cells

AUTHORS

Javad Ranjbari 1 , Abbas Alibakhsh 1 , Roghayeh Arezumand 1 , Mohammad Pourhassan-Moghaddam 1 , Mohammad Rahmati 1 , Nosratollah Zarghami 2 , * , Mohammad Mehdi Namvaran 3

1 Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran

2 Tuberculosis and Lung Research Center, Department of Medical Bi otechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran

3 Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran

How to Cite: Ranjbari J, Alibakhsh A, Arezumand R, Pourhassan-Moghaddam M, Rahmati M, et al. Effects of Curcuma longa Extract on Telomerase Activity in Lung and Breast Cancer Cells, Zahedan J Res Med Sci. 2014 ; 16(10):-.

ARTICLE INFORMATION

Zahedan Journal of Research in Medical Sciences: 16 (10)
Published Online: September 03, 2013
Article Type: Research Article
Received: June 01, 2013
Accepted: August 02, 2013

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Abstract

Background: The purpose of this study is to evaluate the effect of Curcuma longa extract on the telomerase gene expression in QU-DB lung cancer and T47D breast cancer cell lines.

Materials and Methods: The present study is an experimental research. Using 3 different phases n-hexane, dichloromethane and methanol, total extract of Curcuma longa in a serial dilution was prepared and three phases was analyzed for determining which phase has more curcuminoids. Then the extract cytotoxicity effect was tested on breast cancer cell line (T47D), and lung cancer cell line (QU-DB) by 24, 48 and 72 h MTT (Dimethyl thiazolyl diphenyl tetrazolium) assay. Then, the cells were treated with serial concentrations of the extract. Finally, total protein was extracted from the control and test groups, its quantity was determined and telomeric repeat amplification protocol (TRAP) assay was performed for measurement of possible inhibition of the telomerase activity.

Results: Cell viability and MTT-based cytotoxicity assay show that the total extract of Curcuma longa has cytotoxic effect with different IC50s in breast and lung cancer cell lines. Analysis of TRAP assay also shows a significant reduction in telomerase activity on both cancer cells with different levels.

Conclusion: Curcuma longa extract has anti-proliferation and telomerase inhibitory effects on QU-DB lung cancer and T47D breast cancer cells with differences in levels of telomerase inhibition.

Keywords

Telomerase Breast Cancer Lung Cancer Curcuma Longa

© 0, Zahedan Journal of Research in Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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