Evaluation of Antibacterial Activity of Satureja Khuzestanica J. Essential Oil against Standard and Isolated Strains of Listeria monocytogenes

AUTHORS

Khosro Issazadeh 1 , Soheil Shokri-Fashtali 2 , Naser Ghaemi 3 , Alireza Massiha 3 , Mehdi Assmar 3 , Sheida Akbari-Shahabi 3 , *

2 Department of Mathematics, Lahijan Branch , Islamic Azad University, Lahijan, Iran

3 Department of Biotechnology, Tehran University, Tehran, Iran

3 Department of Microbiology, Lahijan Branch, Islamic Azad University, Lahijan, Iran

How to Cite: Issazadeh K, Shokri-Fashtali S, Ghaemi N, Massiha A, Assmar M, et al. Evaluation of Antibacterial Activity of Satureja Khuzestanica J. Essential Oil against Standard and Isolated Strains of Listeria monocytogenes, Zahedan J Res Med Sci. 2014 ; 16(10):-.

ARTICLE INFORMATION

Zahedan Journal of Research in Medical Sciences: 16 (10)
Published Online: June 29, 2013
Article Type: Research Article
Received: December 12, 2012
Accepted: February 10, 2013

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Abstract

Background: The purpose of this study was to determine antibacterial activity of essential oil of Satureja khuzestanica against Listeria monocytogenes (PTCC1295) and strains isolated from breast milk show that.

Materials and Methods: In this descriptive-analytic study, Essence of leave’s plant was extracted and identified its compounds and then carvacrol was isolated. Antibacterial activities were examined by agar dilution method against L. monocytogenes. Minimum inhibitory concentration (MIC) and minimum bacterial concentration (MBC) were carried out by micro dilution method. Then bacterial suspension injected the BALB/c mice. Forty-eight h after seeing the listeriosis disease signs were started the treatment. Ampicillin (10 μg/disc) and trimethoprim (5 g) were used as controls

Results: The results showed that the inhibitory zone diameter standard and essential oils for strains isolated species were respectively 59 and 50 mm. This amount was determined by carvacrol, respectively, 60 and 48 mm. Inhibition zone diameter measurements for standard strains of ampicillin and trimethoprim tedious strains, respectively, 21, 40, 18 and 33 mm, respectively. The minimum inhibitory concentration of essential oils, carvacrol and ampicillin than standard strains, respectively 1.56, 1.56 and 155×10Ë—8 μg/mL and MBC 3.125, 3.125 and 125×10-7 μg/mL was determined by the ratio of the strain 3.125, 3.125 and 0.0062 μg/mL and MBC was 6.25, 6.25 and 0.025 μg/mL.

Conclusion: This study showed that bacterial cleansing properties of essential oil of this plant have a strong and effective combination that is carvacrol.

Keywords

Essential Oils Carvacrol Satureja Khuzestanica Antimicrobial Activity Listeria Monocytogene

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