Phytochemical Analysis, Antibacterial Activity of Marrubium vulgare L against Staphylococcus aureus in vitro


Hamde Khaje 1 , Saphora Bazi 1 , Negar Amini-Borojeni 2 , Abbas Ali Niazi 3 , Mohammad Bokaeian 4 , Elham Saboori 5 , Saeide Saeidi 6 , *


1 Department of Biology, Faculty of Sciences, Payam-e-Noor University, Isfahan, Iran

2 Agricultural Biotechnology Research Institute, Zabol University, Zabol, Iran

3 Infectious Diseases and Tropical Medicine Research Center, Zahedan University of Medical Sciences, Zahedan, Iran

4 Infectious Diseases and Tropical Medicine Research Center, Zahedan University of Medical Sciences,, Zahedan, Iran

5 Department of Agricultural Biotechnology, Zabol University, Zabol, Iran

6 Saeide Saeidi, Department of Biology, Faculty of Sciences, Science and Research Branch, Islamic Azad University, Kerman, Iran


Zahedan Journal of Research in Medical Sciences: 16 (10); 60-64
Published Online: September 10, 2013
Article Type: Research Article
Received: July 02, 2013
Accepted: July 20, 2013




Background:Herbal medicines are the major remedy in traditional medical systems and made a great contribution in maintaining human health and in preventing many infectious diseases. The present study was carried out to determine the potential antibacterial effect of ethanol extracts and essential oil of Marrubium vulgare L. against Staphylococcus aureus which is antibiotic resistant.

Materials and Methods:All 17 strains of S. aureus isolated from nose and throat sample from 160 healthy subjects, hospital staffs and inpatient in the city of Zabol (Amir Al-Momenin hospital, Zabol, south-eastern Iran) were screened during years 2010-2011. In this study, the essential oil of Marrubium vulgare L. obtained by hydrodistillation was analyzed by gas chromatography coupled to mass spectrometry (GC-MS) in order to determine their chemical composition. The minimum inhibitory concentrations were investigated to characterize the antimicrobial activities of this essential oil and extract.

Results:Thirty-one components in the oil of Marrubium vulgare were identified. The results demonstrated that the major components of the essential oil were γ-Eudesmol (11%), Germacrene (10%), D-Citronelly formate (10%), β-Citronellol (8%), Geranyl tiglate (7.1%), Geranyl formate (6.02%). The least MIC value of extract M. vulgare was 2.5 mg/mL and the highest MIC value of essential oil M. vulgare was 2.5 mg/mL.

Conclusion:This investigation showed that the M. vulgare essential oil and extract has a potent antimicrobial activity against S. aureus. The present studies confirm the use of this essential oil and extract as antibacterial agent. Further research is required to evaluate the practical values of therapeutic applications.


Phytochemical Analysis Antibacterial Activity Marrubium Vulgare L

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