Antimicrobial Susceptibility and Distribution of TEM and CTX-M Genes among ESBL-producing Klebsiella pneumoniae and Pseudomonas aeruginosa Causing Urinary Tract Infections

AUTHORS

Saeide Saeidi 1 , Roya Alavi-Naini 2 , * , Sara Shayan 3

AUTHORS INFORMATION

1 Department of Biology, Faculty of Sciences, Islamic Azad University, Kerman, Iran

2 Infectious Diseases and Tropical Medicine Research Center, Zahedan University of Medical Sciences, Zahedan, Iran

3 Department of Microbiology, Faculty of Medicine Sciences, Zahedan University of Medical Sciences, Zahedan, Iran

ARTICLE INFORMATION

Zahedan Journal of Research in Medical Sciences: 16 (4); 1-5
Published Online: May 15, 2013
Article Type: Research Article
Received: March 16, 2015
Accepted: April 17, 2013

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Abstract

Background: Extended spectrum beta lactamases (ESBLs) have been observed in nearly all the species of family Enterobacteriaceae. The enzymes are plasmid mediated and are derived from broad-spectrum beta lactamase TEM and CTX- M by a limited number of mutations. This study was undertaken to characterize ESBL producers among Klebsiella pneumoniae and Pseudomonas aeruginosa by PCR, which were initially screened by phenotypic method.

Materials and Methods: A cross-sectional study was performed to evaluate 180 strains (30 K. pneumoniae and 150 P. aeruginosa) isolated from urine culture of hospitalized patients (Amir Al-Momenin Hospital, Zabol, south-eastern Iran) suffered from urinary tract infections during a period of six months. The prevalence of ESBL producing K. pneumoniae and P. aeruginosa was evaluated by disk diffusion test and polymerase chain reaction (PCR) by detecting TEM and CTX-M gene.

Results: The results of the study revealed that the prevalence of ESBL producing P. aeruginosa and K. pneumoniae by disk diffusion test was 13.3% for P. aeruginosa and 66.6% for K. pneumoniae. Seventy five percent and 65% of K. pneumoniae harboured the gene TEM and CTX-M, respectively. Forty five percent of P. aeruginosa isolates harboured the gene TEM but none of them demonstrated the gene CTX-M using PCR method.

Conclusion: ESBL producing P. aeruginosa and K. pneumoniae isolates showed a high prevalence in this study. Therefore it seems that continuous surveillance is essential to monitor the ESBLs producing microorganisms in hospitals and community.

Keywords

Klebsiella Pneumoniae Pseudomonas Aeruginosa Extended Spectrum Beta-Lactamase TEM Gene CTX-M Gene PCR

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