Detection of Enterotoxigenic Staphylococcus aureus in Schizothorax zarudnyi Using PCR Method

AUTHORS

Narges Ahani 1 , * , Majid Alipour - Eskandani 2

1 Student of master of science of genetics, University of Zabol, Zabol, Iran

2 D epartment of F ood S afety , Faculty of Veterinary, University of Zabol, Zabol, Iran

How to Cite: Ahani N , Alipour - Eskandani M. Detection of Enterotoxigenic Staphylococcus aureus in Schizothorax zarudnyi Using PCR Method, Zahedan J Res Med Sci. 2014 ; 16(4):29-31.

ARTICLE INFORMATION

Zahedan Journal of Research in Medical Sciences: 16 (4); 29-31
Published Online: March 06, 2013
Article Type: Short Communication
Received: January 09, 2013
Accepted: February 05, 2013

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Abstract

Background: Enterotoxins of Staphylococcus aureus are the main factors which cause food poisoning.

Matrrials and Methods: Thirty five samples of Schizothorax zarudnyi were cultured by bacteriological methods and S. aureuses were identified. The isolated bacteria were evaluated by PCR for diagnosis of the gene encoding of SEA and SEB. Data were analyzed using Stata software.

Results: PCR is a rapid, sensitive, specific and inexpensive method for detecting Staphylococcal enterotoxins in food.

Conclusion: 37.2% of fishes were contaminated by S. aureus. The PCR results showed that 14.3% of S. aureus isolates possessed the SEA gene, 8.5% had the SEB gene and 5.7% possessed both genes.

Keywords

Staphylococcus Aureus Enterotoxin PCR

© 0, Zahedan Journal of Research in Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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