Cytotoxic Effect of Aqueous and Alcoholic Extracts of Pterocarya fraxinifolia Leaves on K562 Cell Line

AUTHORS

Zahra Abedigheshlaghi 1 , * , Ramesh Monajemi, 2 , Sima Yahyaabadi 3

AUTHORS INFORMATION

1 Department of Biology, Falavarjan Branch, Islamic Azad University, Isfahan, Andorra

2 Department of Biology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran

3 Department of Biology, Falavarjan Branch, Islamic Azad University, Esfahan, Andorra

ARTICLE INFORMATION

Zahedan Journal of Research in Medical Sciences: 16 (3); 1-5
Published Online: April 10, 2013
Article Type: Research Article
Received: March 17, 2015
Accepted: October 20, 2013

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Abstract

Background: Chronic myeloid leukemia (CML) is a malignant clonal disorder of hematopoietic stem cells which results in increase of myeloid cells, erythroid cells and platelets in the peripheral blood and hyperplasia in bone marrow. Pterocarya fraxinifolia; (Juglandaceae) is widely distributed in northern area of Iran. The research evaluates the cytotoxic effect of n-butanol fraction, aqueous, methanolic and ethanolic extracts of P. fraxinifolia leaves on K562 cell line as a model of chronic myeloid leukemia.

Materials and Methods: Leaves of P. fraxinifolia collected from Astara city and extraction using soxhlet method. K562 cells were cultured and treated with concentrations of extracts (12.5-400 µg/ml). Cytotoxicity of P. fraxinifolia extracts against K562 leukemia cells was estimated by the MTT test method. The absorbance was measured using an ELISA plate reader at 540 nm.

Results: Ethanolic extract showed the highest cytotoxic effect (IC50=148.66±11.64 µg/ml) whereas n-butanol fraction extract were least cytotoxic effect (IC50=248.97±6.71 µg/ml) among the extracts. Aqueous and methanol extracts showed the cytotoxic effect with the IC50=183.14±4.71 and 226.02±6.08 µg/ml on K562 cell line. Both ethanolic and n-butanol fraction extracts exhibited a dose-dependent cytotoxic effect on K562 cell line.

Conclusions: Considering the cytotoxic effects aqueous and alcoholic extracts of P. fraxinifolia leaves on K562 cells, the plant can be considered as a potential candidate for further studies on CML treatment.

Keywords

Pterocarya Fraxinifolia Cytotoxic Leukemia

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