Effect of Saffron aqua Extract on Angiogenesis in Chick Chorioalantoic Membrane

AUTHORS

Marzieh Mousavi 1 , Javad Baharara 2 , * , Saeideh Zafar - Balanezhad 3 , Khadijeh Shaheokh - Abadi 4

1 55 Zahedan Journal of Research in Medical Sciences Journal homepage: www.zjrms.ir Effect of Saffron aqua Extract on Angiogenesis in Chick Chorioalantoic Membrane M arzieh Mo u savi, 1 Javad Baharara, * 1 S aeideh Zafar - Balanezhad, 1 Kh adijeh Shaheokh - Abadi 1 1 . Dep artmen t of Biology , Faculty o f Science s , Applied Biology Center , Mashhad Branch, Islamic Azad University, Mashhad , Iran Article information Abstract Article history: Received: 4 Aug 2012 Accepted: 12 Dec 2012 Available online: 5 May 201 3 ZJRMS 201 4 ; 1 6 ( 3 ): 55 - 58 Background : S tudies confirmed anticancer properties of saffron extract . Angiogenesis, formation of new blood vessels which is necessary in many physiological stages and pathological events such as tumor growth. So it would be an effective strategy to inhibit angiogenesis to treat many cancers and metastasis. In this experimental study , effects of saffron on angiogenesis in chick chorioalantoic membrane (CAM) were investigated . Materials and Methods : Fifity r oss fertilized eggs divided in 5 groups, including : control, sham exposed, experimental group 1, 2 and 3 . In second day of incubation window was opened on eggs . I n day 8 gelatin sponges contain gelatin and albumin was put on chorioalantoic membrane and was soaked with Saffron aqua extract in concentration 100, 400 and 800 μg/ml . In 12th day all cases were photographed by photo stereomicroscope . N umbers and lengths of vessels around the sponges were measured by Image J software . Data were analyzed with SPSS - 16 in significant le vel p <0.05 . Results : According to data analysis, changes had no correlation on the average length of blood vessels in the first experimental group (41.5±5.5 mm), compared with the control group, (44.5±2.4 mm). While in the second and third experimental group (40.2±2.1 mm) and (38.4±3.8 mm) these changes were significant ( p = 0.001). On the other hand, the average number of blood vessels in the first experimental group (22.07±5.2) in compare with the control group (27.46±4.4) shows a significant reduction ( p = 0.0 2 ), this decline between the second (18.80±4.4) and third (15.87±3.8) experimental groups was significant at the level of p = 0.001 . Conclusion : S affron extract has a dose dependent inhibitory effect on angiogenesis in chick chorioalantoic membrane . Copyright © 201 4 Zahedan University of Medical Sciences. All rights reserved. Keywords: Angiogenesis Saffron Cancer * Corresponding author at: Department of Biology, Faculty of Science and Applied Biology Center , Mashhad Branch, Islamic Azad University, Mashhad, Iran

2

3 Dep artmen t of Biology , Faculty o f Science s , Applied Biology Center , Mashhad Branch, Islamic Azad University, Mashhad , Mashhad , Iran

4 Dep artmen t of Biology , Faculty o f Science s , Applied Biology Center , Mashhad Branch, Islamic Azad University, Mashhad, Iran

How to Cite: Mousavi M, Baharara J , Zafar - Balanezhad S, Shaheokh - Abadi K. Effect of Saffron aqua Extract on Angiogenesis in Chick Chorioalantoic Membrane , Zahedan J Res Med Sci. 2014 ; 16(3):55-58.

ARTICLE INFORMATION

Zahedan Journal of Research in Medical Sciences: 16 (3); 55-58
Published Online: May 05, 2013
Article Type: Research Article
Received: April 12, 2012
Accepted: December 12, 2012

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Abstract

Background: Studies confirmed anticancer properties of saffron extract. Angiogenesis, formation of new blood vessels which is necessary in many physiological stages and pathological events such as tumor growth. So it would be an effective strategy to inhibit angiogenesis to treat many cancers and metastasis. In this experimental study, effects of saffron on angiogenesis in chick chorioalantoic membrane (CAM) were investigated.

Materials and Methods: Fifity ross fertilized eggs divided in 5 groups, including: control, sham exposed, experimental group 1, 2 and 3. In second day of incubation window was opened on eggs. In day 8 gelatin sponges contain gelatin and albumin was put on chorioalantoic membrane and was soaked with Saffron aqua extract in concentration 100, 400 and 800 µg/ml. In 12th day all cases were photographed by photo stereomicroscope. Numbers and lengths of vessels around the sponges were measured by Image J software. Data were analyzed with SPSS-16 in significant level p<0.05.

Results: According to data analysis, changes had no correlation on the average length of blood vessels in the first experimental group (41.5±5.5 mm), compared with the control group, (44.5±2.4 mm). While in the second and third experimental group (40.2±2.1 mm) and (38.4±3.8 mm) these changes were significant (p=0.001). On the other hand, the average number of blood vessels in the first experimental group (22.07±5.2) in compare with the control group (27.46±4.4) shows a significant reduction (p=0.02), this decline between the second (18.80±4.4) and third (15.87±3.8) experimental groups was significant at the level of p=0.001.

Conclusion: Saffron extract has a dose dependent inhibitory effect on angiogenesis in chick chorioalantoic membrane.

Keywords

Angiogenesis Saffron Cancer

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