Zahedan Journal of Research in Medical Sciences

Published by: Kowsar

Expression Optimizing and Purification of Recombinant Human Leukemia Inhibitory Factor Produced in E. coli Strain BL21

Houman Kahroba 1 , Hamid Galehdari 1 , * , Mohammolad Shafeei 1 , Saeed Reza Khatami 1 and Ali Khodadadi 2
Authors Information
1 Department of Genetics, Shahid Chamran University, Ahvaz, Iran
2 Cancer, Petroleum and Environmental Pollutants Research Center, Jundishapur University of Medical Sciences, Ahvaz, Iran
Article information
  • Zahedan Journal of Research in Medical Sciences: February 28, 2015, 17 (2); e1998
  • Published Online: January 29, 2014
  • Article Type: Research Article
  • Received: July 30, 2013
  • Accepted: October 19, 2013

To Cite: Kahroba H, Galehdari H, Shafeei M, Khatami S R, Khodadadi A. Expression Optimizing and Purification of Recombinant Human Leukemia Inhibitory Factor Produced in E. coli Strain BL21, Zahedan J Res Med Sci. 2015 ; 17(2):e1998.

Abstract
Copyright © 2015, Zahedan University of Medical Sciences. All rights reserved.This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
Introduction
Materials and Methods
Results
Discussion
Acknowledgements
Footnotes
References
  • 1. Dimitriadis E, Salamonsen LA, Menkhorst E. Leukemia inhibitory factor and human endometrial receptivity. Indian J Physiol Pharmacol. 2010; 54(5): 17-26
  • 2. Auernhammer CJ, Melmed S. Leukemia-inhibitory factor-neuroimmune modulator of endocrine function. Endocr Rev. 2000; 21(3): 313-45[DOI][PubMed]
  • 3. Gearing DP, Gough NM, King JA, Hilton DJ, Nicola NA, Simpson RJ, et al. Molecular cloning and expression of cDNA encoding a murine myeloid leukaemia inhibitory factor (LIF). EMBO J. 1987; 6(13): 3995-4002[PubMed]
  • 4. Ikeda K, Iwasaki Y, Tagaya N, Shiojima T, Kinoshita M. Neuroprotective effect of cholinergic differentiation factor/leukemia inhibitory factor on wobbler murine motor neuron disease. Muscle Nerve. 1995; 18(11): 1344-7[DOI][PubMed]
  • 5. Rahim F, Saki G. Assessment of Motility and Survival Rate of Asthenospermic Men's Sperm Cultured in Media Plus Leukemia Inhibitor Factor. Int J Pharmacol. 2010; 6(3): 306-10[DOI]
  • 6. Cheema SS, Richards LJ, Murphy M, Bartlett PF. Leukaemia inhibitory factor rescues motoneurones from axotomy-induced cell death. NeuroReport. 1994; 5(8): 989-92[DOI]
  • 7. Kurek JB, Austin L, Cheema SS, Bartlett PF, Murphy M. Up-regulation of leukaemia inhibitory factor and interleukin-6 in transected sciatic nerve and muscle following denervation. Neuromuscular Disorders. 1996; 6(2): 105-14[DOI]
  • 8. Chen JR, Cheng JG, Shatzer T, Sewell L, Hernandez L, Stewart CL. Leukemia inhibitory factor can substitute for nidatory estrogen and is essential to inducing a receptive uterus for implantation but is not essential for subsequent embryogenesis. Endocrinology. 2000; 141(12): 4365-72[DOI][PubMed]
  • 9. Tjernlund A. [Leukemia inhibitory factor (LIF) and gp130 in early defence against HIV-1 infection] [dissertation]. 2006;
  • 10. Metcalf D, Nicola NA, Gearing DP. Effects of injected leukemia inhibitory factor on hematopoietic and other tissues in mice. Blood. 1990; 76(1): 50-6[PubMed]
  • 11. Mayer P, Geissler K, Ward M, Metcalf D. Recombinant human leukemia inhibitory factor induces acute phase proteins and raises the blood platelet counts in nonhuman primates. Blood. 1993; 81(12): 3226-33[PubMed]
  • 12. Hilton DJ. LIF: lots of interesting functions. Trends in Biochemical Sciences. 1992; 17(2): 72-6[DOI]
  • 13. Mikolajczyk M, Skrzypczak J, Szymanowski K, Wirstlein P. The assessment of LIF in uterine flushing--a possible new diagnostic tool in states of impaired fertility. Reprod Biol. 2003; 3(3): 259-70[PubMed]
  • 14. Richter KS. The importance of growth factors for preimplantation embryo development and in-vitro culture. Curr Opin Obstet Gynecol. 2008; 20(3): 292-304[DOI][PubMed]
  • 15. Dimitriadis E, Menkhorst E, Salamonsen LA, Paiva P. Review: LIF and IL11 in trophoblast-endometrial interactions during the establishment of pregnancy. Placenta. 2010; 31 Suppl-104[DOI][PubMed]
  • 16. Darabi P, Galehdari H, Khatami SR, Shahbazian N, Shafeei M, Jalali A, et al. Codon Optimization, Cloning and Expression of the Human Leukemia Inhibitory Factor (hLIF) in E. coli. Iranian Journal of Biotechnology. 2012; 11(1): 47-53[DOI]
  • 17. Green MR, Sambrook J. Molecular cloning a laboratory manual. 2012;
  • 18. Imsoonthornruksa S, Noisa P, Parnpai R, Ketudat-Cairns M. A simple method for production and purification of soluble and biologically active recombinant human leukemia inhibitory factor (hLIF) fusion protein in Escherichia coli. J Biotechnol. 2011; 151(4): 295-302[DOI][PubMed]
  • 19. Gough NM, Gearing DP, King JA, Willson TA, Hilton DJ, Nicola NA, et al. Molecular cloning and expression of the human homologue of the murine gene encoding myeloid leukemia-inhibitory factor. Proc Natl Acad Sci U S A. 1988; 85(8): 2623-7[PubMed]
  • 20. Horiuchi H, Tategaki A, Yamashita Y, Hisamatsu H, Ogawa M, Noguchi T, et al. Chicken leukemia inhibitory factor maintains chicken embryonic stem cells in the undifferentiated state. J Biol Chem. 2004; 279(23): 24514-20[DOI][PubMed]
  • 21. Tomala M, Lavrentieva A, Moretti P, Rinas U, Kasper C, Stahl F, et al. Preparation of bioactive soluble human leukemia inhibitory factor from recombinant Escherichia coli using thioredoxin as fusion partner. Protein Expr Purif. 2010; 73(1): 51-7[DOI][PubMed]
  • 22. Cui S, Selwood L. cDNA cloning, characterization, expression and recombinant protein production of leukemia inhibitory factor (LIF) from the marsupial, the brushtail possum (Trichosurus vulpecula). Gene. 2000; 243(1-2): 167-78[DOI]
  • 23. Abe T, Mikekado T, Haga S, Kisara Y, Watanabe K, Kurokawa T, et al. Identification, cDNA cloning, and mRNA localization of a zebrafish ortholog of leukemia inhibitory factor. Comp Biochem Physiol B Biochem Mol Biol. 2007; 147(1): 38-44[DOI][PubMed]
  • 24. Song JH, Houde A, Murphy BD. Cloning of leukemia inhibitory factor (LIF) and its expression in the uterus during embryonic diapause and implantation in the mink (Mustela vison). Mol Reprod Dev. 1998; 51(1): 13-21[DOI][PubMed]
  • 25. Quesenberry PJ, Morley A, Ryan M, Howard D, Stohlman FJ. The effect of endotoxin on murine stem cells. J Cell Physiol. 1973; 82(2): 239-44[DOI][PubMed]
  • 26. Quesenberry P, Morley A, Stohlman FJ, Rickard K, Howard D, Smith M. Effect of endotoxin on granulopoiesis and colony-stimulating factor. N Engl J Med. 1972; 286(5): 227-32[DOI][PubMed]
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