Zahedan Journal of Research in Medical Sciences

Published by: Kowsar

Comparison of Direct Immunofluorescence and Giemsa Staining in Chlamydia trachomatis Follicular Conjunctivitis

Fahimeh Asadi-Amoli 1 , * , Zohreh Nozarian 1 , Vahid Mehrtash 2 , Hooshang Beheshtnejad 3 and Avishan Shabani 4
Authors Information
1 Department of Pathology, Tehran University of Medical Sciences, Tehran, IR Iran
2 Student of Medicine, School of Medicine, Tehran University of Medical Sciences, Tehran, IR Iran
3 Department of Ophthalmology, Tehran University of Medical Sciences, Tehran, IR Iran
4 Department of Pharmacology, Tehran University of Medical Sciences, Tehran, IR Iran
Article information
  • Zahedan Journal of Research in Medical Sciences: October 28, 2015, 17 (10); e2181
  • Published Online: October 25, 2015
  • Article Type: Research Article
  • Received: November 5, 2011
  • Accepted: December 22, 2011
  • DOI: 10.17795/zjrms-2181

To Cite: Asadi-Amoli F, Nozarian Z, Mehrtash V, Beheshtnejad H, Shabani A. Comparison of Direct Immunofluorescence and Giemsa Staining in Chlamydia trachomatis Follicular Conjunctivitis, Zahedan J Res Med Sci. 2015 ; 17(10):e2181. doi: 10.17795/zjrms-2181.

Copyright © 2015, Zahedan University of Medical Sciences.This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Patients and Methods
4. Results
5. Discussion
  • 1. Mahon CR, Lehman DC, Manuselis G. Textbook of diagnostic microbiology. 2010; : 551-6
  • 2. Gallo Vaulet L, Entrocassi C, Corominas AI, Rodriguez Fermepin M. Distribution study of Chlamydia trachomatis genotypes in symptomatic patients in Buenos Aires, Argentina: association between genotype E and neonatal conjunctivitis. BMC Res Notes. 2010; 3: 34[DOI][PubMed]
  • 3. Spenser WH. Ophthalmic pathology: An atlas and textbook. 1996; : 71-3
  • 4. Yanoff M, Sassani JW. Ocular pathology. 2014; : 231-2
  • 5. Tille PM. Bailey& Scott diagnostic microbiology. 2013; : 515-9
  • 6. Joesoef MR, Weinstock HS, Johnson RE. Factors associated with recurrent chlamydial infection and failure to return for retesting in young women entering national job training program, 1998--2005. Sex Transm Dis. 2008; 35(4): 368-71[DOI][PubMed]
  • 7. Meyer T. [Modern diagnosis of Chlamydia trachomatis infections]. Hautarzt. 2007; 58(1): 24-30[DOI][PubMed]
  • 8. Tullo AB, Richmond SJ, Easty DL. The presentation and incidence of paratrachoma in adults. J Hyg (Lond). 1981; 87(1): 63-9[PubMed]
  • 9. Rours IG, Hammerschlag MR, Ott A, De Faber TJ, Verbrugh HA, de Groot R, et al. Chlamydia trachomatis as a cause of neonatal conjunctivitis in Dutch infants. Pediatrics. 2008; 121(2)-6[DOI][PubMed]
  • 10. Epidemiological study of neonatal conjunctivitis. Proceeding of the 24th international congress of pediatrics.
  • 11. Di Bartolomeo S, Mirta DH, Janer M, Fermepin MR, Sauka D, Magarinos F, et al. Incidence of Chlamydia trachomatis and other potential pathogens in neonatal conjunctivitis. Int J Infect Dis. 2001; 5(3): 139-43
  • 12. Haller EM, Auer-Grumbach P, Stuenzner D, Kessler HH, Pierer K, Zenz H, et al. Evaluation of two nonculture antigen tests and three serotests for detection of anti-chlamydial antibodies in the diagnosis of ocular chlamydial infections. Graefes Arch Clin Exp Ophthalmol. 1996; 234(8): 510-4[PubMed]
  • 13. Lin J, Li Y, Zhang J, Feng G, Zhang P, Zheng H, et al. Rapid diagnosis of chlamydial conjunctivitis in laboratory. Yan Ke Xue Bao. 1999; 15(3): 191-4[PubMed]
  • 14. Bashour M. Ophthalmologic manifestations of Chlamydia. 2014;
  • 15. Mcpherso RA, Pincus MR. Henrys clinical diagnosis and management by laboratory methods. 2012; : 1066-9
  • 16. Chandran L, Boykan R. Chlamydial infections in children and adolescents. Pediatr Rev. 2009; 30(7): 243-50[DOI][PubMed]
  • 17. Watson EJ, Templeton A, Russell I, Paavonen J, Mardh PA, Stary A, et al. The accuracy and efficacy of screening tests for Chlamydia trachomatis: a systematic review. J Med Microbiol. 2002; 51(12): 1021-31[DOI][PubMed]
  • 18. Olshen E, Shrier LA. Diagnostic tests for chlamydial and gonorrheal infections. Semin Pediatr Infect Dis. 2005; 16(3): 192-8[PubMed]
  • 19. Javaloy J, Ferrer C, Vidal MT, Alio JL. Follicular conjunctivitis caused by Chlamydia trachomatis in an infant Saharan population: molecular and clinical diagnosis. Br J Ophthalmol. 2003; 87(2): 142-6[PubMed]
  • 20. Taylor-Robinson D, Thomas BJ. Laboratory techniques for the diagnosis of chlamydial infections. Genitourin Med. 1991; 67(3): 256-66[PubMed]
  • 21. Palayekar VV, Joshi JV, Hazari KT, Shah RS, Chitlange SM. Comparison of four nonculture diagnostic tests for Chlamydia trachomatis infection. J Assoc Physicians India. 2000; 48(5): 481-3[PubMed]
  • 22. Munday PE, Johnson AP, Thomas BJ, Taylor-Robinson D. A comparison of the sensitivity of immunofluorescence and Giemsa for staining Chlamydia trachomatis inclusions in cycloheximide-treated McCoy cells. J Clin Pathol. 1980; 33(2): 177-9[PubMed]
  • 23. Mellman-Rubin TL, Kowalski RP, Uhrin M, Gordon YJ. Incidence of adenoviral and chlamydial coinfection in acute follicular conjunctivitis. Am J Ophthalmol. 1995; 119(5): 652-4[PubMed]
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:



Create Citiation Alert
via Google Reader

Readers' Comments