Comparison of Culture and Multiplex PCR Technique for Detection of Brucella abortus and Brucella melitensis from Human Blood Samples

AUTHORS

Reza Mirnejad 1 , Ali Reza Vahdati 2 , Ali Ahmadi 1 , Seied Mojtaba Mortazavi 3 , Vahhab Piranfar 4 , *

AUTHORS INFORMATION

1 Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

2 Institute of Evolutionary Biology and Environmental Studies, University of Zurich, Zurich, Switzerland

3 Health Station, Kerman University of Medical Sciences, Kerman, Iran

4 Department of Microbiology, Tonekabon Branch, Islamic Azad University of Tonekabon, Tonekabon, Iran

ARTICLE INFORMATION

Zahedan Journal of Research in Medical Sciences: 15 (12); e4325
Published Online: February 12, 2013
Article Type: Research Article
Received: November 27, 2012
Accepted: January 09, 2013

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Abstract

Background: To compare culture methods with multiplex PCR technique for identification of Brucella abortus and Brucella melitensis from suspicious patients with clinical history of brucellosis and positive serological test (Rose Bengal test and serum agglutination test).

Materials and Methods: In this study, 160 blood samples from patients suspected of Brucellosis with high serum titers of 1/80 were studied. All samples were cultured in Brucella-specific media. Brucella species were identified by using microbiological methods. DNA was extracted with Phenol-chloroform DNA extraction method. IS711 was amplified simultaneously using three specific primers and obtained patterns were analyzed.

Results: From 160 samples, 47.5% (76) were culture positive cases from which 43 cases were B. melitensis and 33 were B. abortus With the PCR technique 108 were detected positive from which 45.3% were B. abortus and 54.6% were B. melitensis. It should be noted that all 76 samples with positive culture were also identified by PCR.

Conclusion: Generally, use of the molecular technique multiplex PCR in addition to increased speed and accuracy and less false results than bacterial culture method, is able to identify different species of brucella. This will facilitate the treatment process.

Keywords

Brucella abortus Brucella melitensis Multiplex PCR Culture method

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