Prevalence of Campylobacter Jejuni and Coli in Sheep Carcasses by Using Cultural and PCR Methods

AUTHORS

Reza Shahrokhabadi 1 , * , Ebrahim Rahimi 2 , Hassan Mommtaz 3 , Rahele Poursahebi 4

AUTHORS INFORMATION

1 Young Researchers Club, Shahr-e-Kord Branch, Islamic Azad University, Shahr-e-Kord, Iran

2 Department of Food Hygiene, Faculty of Veterinary Medicine, Shahr-e-Kord Branch, Islamic Azad University, Shahr-e-Kord, Iran

3 Department of Microbiology, Faculty of Veterinary Medicine, Shahr-e-Kord Branch, Islamic Azad University, Shahr-e-Kord, Iran

4 Department of Food Hygiene, Faculty of Veterinary Medicine, Bahonar University, Kerman, Iran

How to Cite: Shahrokhabadi R, Rahimi E, Mommtaz H, Poursahebi R. Prevalence of Campylobacter Jejuni and Coli in Sheep Carcasses by Using Cultural and PCR Methods , Zahedan J Res Med Sci. 2013 ; 15(12):e4331.

ARTICLE INFORMATION

Zahedan Journal of Research in Medical Sciences: 15 (12); e4331
Published Online: February 25, 2013
Article Type: Short Communication
Received: November 15, 2012
Accepted: January 30, 2013

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Abstract

Background: Campylobacter species are common bacterial pathogens causing gastroenteritis in humans worldwide.

Materials and Methods: A total of 148 randomly sheep carcasses were sampled by surface section of neck meat taken immediately after slaughter analyzed using microbiological examinations.

Results: Campylobacter spp. was isolated from 10.13% meat cultures samples examined. Among these 80% sample were C. jejuni and 20% sample were C. coli. Using PCR assays, the number of positive campylobacters increased to 11.48%. Of these positive samples, 82.35% were C. jejuni and 17.65% were C. coli. Significantly higher prevalence rates of Campylobacter spp. (p<0.05) were found in the meat samples taken in summer (47.05%).

Conclusion: The PCR is a reliable and sensitive method which can be used as a diagnostic technique for the detection of campylobacter in lamb samples.

Keywords

Campylobacter jejuni Campylobacter coli PCR Sheep

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