Zahedan Journal of Research in Medical Sciences

Published by: Kowsar

CTX-M-15 Type β-lactamases From Clinical Isolates of Escherichia coli by Polymerase Chain Reaction and DNA Sequencing

Mahboobeh Nakhaei Moghaddam 1 , * , Mohammad Reza Zolfaghari 2 and Niloofar Tavakoli‐Hoseini 3
Authors Information
1 Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, IR Iran
2 Department of Microbiology, Qom Branch, Islamic Azad University, Qom, IR Iran
3 Department of Biochemistry and Clinical Laboratories, Tabriz University of Medical Sciences, Tabriz, IR Iran
Article information
  • Zahedan Journal of Research in Medical Sciences: March 2017, 19 (3); e5814
  • Published Online: March 27, 2017
  • Article Type: Research Article
  • Received: February 27, 2016
  • Accepted: March 20, 2017
  • DOI: 10.5812/zjrms.5814

To Cite: Nakhaei Moghaddam M, Zolfaghari M R, Tavakoli‐Hoseini N. CTX-M-15 Type β-lactamases From Clinical Isolates of Escherichia coli by Polymerase Chain Reaction and DNA Sequencing, Zahedan J Res Med Sci. 2017 ; 19(3):e5814. doi: 10.5812/zjrms.5814.

Copyright © 2017, Zahedan Journal of Research in Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Methods
3. Results
4. Discussion
  • 1. Nordmann P, Dortet L, Poirel L. Rapid detection of extended-spectrum- -lactamase-producing Enterobacteriaceae. J Clin Microbiol. 2012; 50(9): 3016-22[DOI]
  • 2. Bush K, Jacoby GA. Updated functional classification of beta-lactamases. Antimicrob Agents Chemother. 2010; 54(3): 969-76[DOI][PubMed]
  • 3. Markovska R, Schneider I, Keuleyan E, Sredkova M, Ivanova D, Markova B, et al. Extended-spectrum beta-lactamase-producing Enterobacteriaceae in Bulgarian hospitals. Microb Drug Resist. 2008; 14(2): 119-28[DOI][PubMed]
  • 4. Abraham EP, Chain E. An enzyme from bacteria able to destroy penicillin. Nature. 1940; 146(3713): 837
  • 5. Poirel L, Bonnin RA, Nordmann P. Genetic support and diversity of acquired extended-spectrum beta-lactamases in Gram-negative rods. Infect Genet Evol. 2012; 12(5): 883-93[DOI][PubMed]
  • 6. Rodriguez-Bano J, Pascual A. Clinical significance of extended-spectrum beta-lactamases. Expert Rev Anti Infect Ther. 2008; 6(5): 671-83[DOI][PubMed]
  • 7. Owlia P, Bameri Z, Chitsaz M. Detection of CTX-M-β lactamases form isolated klebsiella pneumoniae. Iranian Journal of Pathology. 2010; 5(3): 137-42
  • 8. Sridhar Rao PN. Extended spectrum beta-lactamases- A comprehensive review. 2015;
  • 9. Sid Ahmed MA, Bansal D, Acharya A, Elmi AA, Hamid JM, Sid Ahmed AM, et al. Antimicrobial susceptibility and molecular epidemiology of extended-spectrum beta-lactamase-producing Enterobacteriaceae from intensive care units at Hamad Medical Corporation, Qatar. Antimicrob Resist Infect Control. 2016; 5: 4[DOI][PubMed]
  • 10. Sullivan R, Schaus D, John M, Delport JA. Extended spectrum beta-lactamases: A minireview of clinical relevant groups. J Med Microb Diagn. 2015; 4(203): 2161-703.1000203
  • 11. Livermore DM, Woodford N. The beta-lactamase threat in Enterobacteriaceae, Pseudomonas and Acinetobacter. Trends Microbiol. 2006; 14(9): 413-20[DOI][PubMed]
  • 12. Tille P. Bailey & Scott's diagnostic microbiology. 2015;
  • 13. Jadhav S, Hussain A, Devi S, Kumar A, Parveen S, Gandham N, et al. Virulence characteristics and genetic affinities of multiple drug resistant uropathogenic Escherichia coli from a semi urban locality in India. PLoS One. 2011; 6(3)[DOI][PubMed]
  • 14. Cockerill FR. Performance standards for antimicrobial susceptibility testing: twenty-first informational supplement. 2011;
  • 15. Saeidi S, Alavi-Naini R, Shayan S. Antimicrobial susceptibility and distribution of tem and ctx-m genes among ESBL-producing Klebsiella pneumoniae and Pseudomonas aeruginosa causing urinary tract infections. Zahedan J Res Med Sci. 2014; 16(4): 1-5
  • 16. M'Zali FH, Chanawong A, Kerr KG, Birkenhead D, Hawkey PM. Detection of extended-spectrum beta-lactamases in members of the family enterobacteriaceae: comparison of the MAST DD test, the double disc and the Etest ESBL. J Antimicrob Chemother. 2000; 45(6): 881-5[PubMed]
  • 17. Philippon A, Arlet G, Jacoby GA. Plasmid-determined AmpC-type beta-lactamases. Antimicrob Agents Chemother. 2002; 46(1): 1-11[PubMed]
  • 18. Pagani L, Dell'Amico E, Migliavacca R, D'Andrea MM, Giacobone E, Amicosante G, et al. Multiple CTX-M-type extended-spectrum beta-lactamases in nosocomial isolates of Enterobacteriaceae from a hospital in northern Italy. J Clin Microbiol. 2003; 41(9): 4264-9[PubMed]
  • 19. Tenover FC. Mechanisms of antimicrobial resistance in bacteria. Am J Med. 2006; 119(6 Suppl 1)-10[DOI][PubMed]
  • 20. Canton R, Coque TM. The CTX-M beta-lactamase pandemic. Curr Opin Microbiol. 2006; 9(5): 466-75[DOI][PubMed]
  • 21. Ruppe E, Hem S, Lath S, Gautier V, Ariey F, Sarthou JL, et al. CTX-M beta-lactamases in Escherichia coli from community-acquired urinary tract infections, Cambodia. Emerg Infect Dis. 2009; 15(5): 741-8[DOI][PubMed]
  • 22. Arpin C, Dubois V, Coulange L, Andre C, Fischer I, Noury P, et al. Extended-spectrum beta-lactamase-producing Enterobacteriaceae in community and private health care centers. Antimicrob Agents Chemother. 2003; 47(11): 3506-14[PubMed]
  • 23. Woodford N, Ward ME, Kaufmann ME, Turton J, Fagan EJ, James D, et al. Community and hospital spread of Escherichia coli producing CTX-M extended-spectrum beta-lactamases in the UK. J Antimicrob Chemother. 2004; 54(4): 735-43[DOI][PubMed]
  • 24. Pitout JD, Hanson ND, Church DL, Laupland KB. Population-based laboratory surveillance for Escherichia coli-producing extended-spectrum beta-lactamases: importance of community isolates with blaCTX-M genes. Clin Infect Dis. 2004; 38(12): 1736-41[DOI][PubMed]
  • 25. Shi H, Sun F, Chen J, Ou Q, Feng W, Yong X, et al. Epidemiology of CTX-M-type extended-spectrum beta-lactamase (ESBL)-producing nosocomial -Escherichia coli infection in China. Ann Clin Microbiol Antimicrob. 2015; 14: 4[DOI][PubMed]
  • 26. Edelstein M, Pimkin M, Palagin I, Edelstein I, Stratchounski L. Prevalence and molecular epidemiology of CTX-M extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae in Russian hospitals. Antimicrob Agents Chemother. 2003; 47(12): 3724-32[PubMed]
  • 27. Peerayeh SN, Eslami M, Memariani M, Siadat SD. High prevalence of blaCTX-M-1 group extended-spectrum β-lactamase genes in Escherichia coli isolates from Tehran. Jundishapur J Microbiol. 2013; 6(7)
  • 28. Iroha IR, Esimone CO, Neumann S, Marlinghaus L, Korte M, Szabados F, et al. First description of Escherichia coli producing CTX-M-15- extended spectrum beta lactamase (ESBL) in out-patients from south eastern Nigeria. Ann Clin Microbiol Antimicrob. 2012; 11: 19[DOI][PubMed]
  • 29. Birgy A, Mariani-Kurkdjian P, Bidet P, Doit C, Genel N, Courroux C, et al. Characterization of extended-spectrum-beta-lactamase-producing Escherichia coli strains involved in maternal-fetal colonization: prevalence of E. coli ST131. J Clin Microbiol. 2013; 51(6): 1727-32
  • 30. Hashemi A. Detection of blactx-m, blatem, blashv genes in Klebsiella pneumoniae strains isolated from two hospitals of Tehran, Iran. Iran J Public Health. 2014; 43(2): 98
  • 31. Xia S, Fan X, Huang Z, Xia L, Xiao M, Chen R, et al. Dominance of CTX-M-type extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli isolated from patients with community-onset and hospital-onset infection in China. PLoS One. 2014; 9(7)[DOI][PubMed]
  • 32. Timofte D, Maciuca IE, Evans NJ, Williams H, Wattret A, Fick JC, et al. Detection and molecular characterization of Escherichia coli CTX-M-15 and Klebsiella pneumoniae SHV-12 beta-lactamases from bovine mastitis isolates in the United Kingdom. Antimicrob Agents Chemother. 2014; 58(2): 789-94[DOI][PubMed]
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