Zahedan Journal of Research in Medical Sciences

Published by: Kowsar

Ex-Vivo Gene Therapy Using Lentiviral Mediated Gene Transfer Into Umbilical Cord Blood Derived Stem Cells

Hanieh Jalali 1 , * , Kazem Parivar 2 , Masoud Soleimani 3 , Mohammad Nabiuni 4 and Hamid Aghaee-Bakhtiari 5
Authors Information
1 Department of Developmental Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, IR Iran
2 Department of Biology, Sciences and Research Branch, Islamic Azad University, Tehran, IR Iran
3 Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IR Iran
4 Department of Cell and Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, IR Iran
5 Department of Molecular Biology and Genetic Engineering, Stem Cells Technology Research Center, Tehran, IR Iran
Article information
  • Zahedan Journal of Research in Medical Sciences: February 2016, 18 (2); e5991
  • Published Online: February 27, 2016
  • Article Type: Research Article
  • Received: September 17, 2014
  • Accepted: November 22, 2014
  • DOI: 10.17795/zjrms-5991

To Cite: Jalali H, Parivar K, Soleimani M, Nabiuni M, Aghaee-Bakhtiari H. Ex-Vivo Gene Therapy Using Lentiviral Mediated Gene Transfer Into Umbilical Cord Blood Derived Stem Cells, Zahedan J Res Med Sci. 2016 ; 18(2):e5991. doi: 10.17795/zjrms-5991.

Copyright © 2016, Zahedan University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
  • 1. Pardridge WM. Molecular Trojan horses for blood-brain barrier drug delivery. Curr Opin Pharmacol. 2006; 6(5): 494-500[DOI][PubMed]
  • 2. Lim ST, Airavaara M, Harvey BK. Viral vectors for neurotrophic factor delivery: a gene therapy approach for neurodegenerative diseases of the CNS. Pharmacol Res. 2010; 61(1): 14-26[DOI][PubMed]
  • 3. Jakobsson J, Ericson C, Rosenqvist N, Lundberg C. Lentiviral vectors. Int Rev Neurobiol. 2003; 55: 111-22[PubMed]
  • 4. Manfredsson FP, Mandel RJ. The development of flexible lentiviral vectors for gene transfer in the CNS. Exp Neurol. 2011; 229(2): 201-6[DOI][PubMed]
  • 5. Snyder EY, Senut MC. The use of nonneuronal cells for gene delivery. Neurobiol Dis. 1997; 4(2): 69-102[DOI][PubMed]
  • 6. Hwang DH, Jeong SR, Kim BG. Gene transfer mediated by stem cell grafts to treat CNS injury. Expert Opin Biol Ther. 2011; 11(12): 1599-610[DOI][PubMed]
  • 7. Lindvall O, Kokaia Z. Stem cells in human neurodegenerative disorders--time for clinical translation? J Clin Invest. 2010; 120(1): 29-40[DOI][PubMed]
  • 8. Roybal JL, Santore MT, Flake AW. Stem cell and genetic therapies for the fetus. Semin Fetal Neonatal Med. 2010; 15(1): 46-51[DOI][PubMed]
  • 9. Hermens WT, Verhaagen J. Viral vectors, tools for gene transfer in the nervous system. Prog Neurobiol. 1998; 55(4): 399-432[PubMed]
  • 10. Snyder BR, Boulis NM, Federici T. Viral vector-mediated gene transfer for CNS disease. Expert Opin Biol Ther. 2010; 10(3): 381-94[DOI][PubMed]
  • 11. Azzouz M, Kingsman SM, Mazarakis ND. Lentiviral vectors for treating and modeling human CNS disorders. J Gene Med. 2004; 6(9): 951-62[DOI][PubMed]
  • 12. Michel G, Yu Y, Chang T, Yee JK. Site-specific gene insertion mediated by a Cre-loxP-carrying lentiviral vector. Mol Ther. 2010; 18(10): 1814-21[DOI][PubMed]
  • 13. Jalali H, Parivar K, Nabiuni M, Soleimani M. Unrestricted somatic stem cells as vehicle for nerve growth factor gene transfer. Neurol Res. 2013; 35(6): 553-60[DOI][PubMed]
  • 14. Kogler G, Sensken S, Airey JA, Trapp T, Muschen M, Feldhahn N, et al. A new human somatic stem cell from placental cord blood with intrinsic pluripotent differentiation potential. J Exp Med. 2004; 200(2): 123-35[DOI][PubMed]
  • 15. Fallahi-Sichani M, Soleimani M, Najafi SM, Kiani J, Arefian E, Atashi A. In vitro differentiation of cord blood unrestricted somatic stem cells expressing dopamine-associated genes into neuron-like cells. Cell Biol Int. 2007; 31(3): 299-303[DOI][PubMed]
  • 16. Rosenkranz K, Meier C. Umbilical cord blood cell transplantation after brain ischemia--from recovery of function to cellular mechanisms. Ann Anat. 2011; 193(4): 371-9[DOI][PubMed]
  • 17. Hafizi M, Bakhshandeh B, Soleimani M, Atashi A. Exploring the enkephalinergic differentiation potential in adult stem cells for cell therapy and drug screening implications. In Vitro Cell Dev Biol Anim. 2012; 48(9): 562-9[DOI][PubMed]
  • 18. Iwaniuk KM, Schira J, Weinhold S, Jung M, Adjaye J, Muller HW, et al. Network-like impact of MicroRNAs on neuronal lineage differentiation of unrestricted somatic stem cells from human cord blood. Stem Cells Dev. 2011; 20(8): 1383-94[DOI][PubMed]
  • 19. Ali H, Bahbahani H. Umbilical cord blood stem cells - potential therapeutic tool for neural injuries and disorders. Acta Neurobiol Exp (Wars). 2010; 70(3): 316-24[PubMed]
  • 20. Gage FH, Fisher LJ, Jinnah HA, Rosenberg MB, Tuszynski MH, Friedmann T. Grafting genetically modified cells to the brain: conceptual and technical issues. Prog Brain Res. 1990; 82: 1-10[PubMed]
  • 21. Vishwakarma SK, Bardia A, Tiwari SK, Paspala SA, Khan AA. Current concept in neural regeneration research: NSCs isolation, characterization and transplantation in various neurodegenerative diseases and stroke: A review. J Adv Res. 2014; 5(3): 277-94[DOI][PubMed]
  • 22. Cunningham LA, Su C. Astrocyte delivery of glial cell line-derived neurotrophic factor in a mouse model of Parkinson's disease. Exp Neurol. 2002; 174(2): 230-42[DOI][PubMed]
  • 23. Kogler G, Radke TF, Lefort A, Sensken S, Fischer J, Sorg RV, et al. Cytokine production and hematopoiesis supporting activity of cord blood-derived unrestricted somatic stem cells. Exp Hematol. 2005; 33(5): 573-83[DOI][PubMed]
  • 24. Trompeter HI, Abbad H, Iwaniuk KM, Hafner M, Renwick N, Tuschl T, et al. MicroRNAs MiR-17, MiR-20a, and MiR-106b act in concert to modulate E2F activity on cell cycle arrest during neuronal lineage differentiation of USSC. PLoS One. 2011; 6(1)[DOI][PubMed]
  • 25. Schira J, Gasis M, Estrada V, Hendricks M, Schmitz C, Trapp T, et al. Significant clinical, neuropathological and behavioural recovery from acute spinal cord trauma by transplantation of a well-defined somatic stem cell from human umbilical cord blood. Brain. 2012; 135: 431-46[DOI][PubMed]
  • 26. Trapp T, Kogler G, El-Khattouti A, Sorg RV, Besselmann M, Focking M, et al. Hepatocyte growth factor/c-MET axis-mediated tropism of cord blood-derived unrestricted somatic stem cells for neuronal injury. J Biol Chem. 2008; 283(47): 32244-53[DOI][PubMed]
  • 27. Washbourne P, McAllister AK. Techniques for gene transfer into neurons. Curr Opin Neurobiol. 2002; 12(5): 566-73[PubMed]
  • 28. Trono D. Lentiviral vectors: turning a deadly foe into a therapeutic agent. Gene Ther. 2000; 7(1): 20-3[DOI][PubMed]
  • 29. Staunstrup NH, Moldt B, Mates L, Villesen P, Jakobsen M, Ivics Z, et al. Hybrid lentivirus-transposon vectors with a random integration profile in human cells. Mol Ther. 2009; 17(7): 1205-14[DOI][PubMed]
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