Zahedan Journal of Research in Medical Sciences

Published by: Kowsar

A Simple Method for High-Level Expression of HTLV-1 gp46-I(162-214) Peptide and Its Application for Screening

Shahrzad Behdad 1 , Samaneh Dolatabadi 1 , * and Jafar Saeidi 2
Authors Information
1 Department of Microbiology, Faculty of Sciences, Neyshabur Branch, Islamic Azad University, Neyshabur, Khorasan Razavi, IR Iran
2 Department of Biology, Faculty of Sciences, Neyshabur Branch, Islamic Azad University, Neyshabur, Khorasan Razavi, IR Iran
Article information
  • Zahedan Journal of Research in Medical Sciences: February 2018, 20 (2); e60736
  • Published Online: February 28, 2018
  • Article Type: Research Article
  • Received: August 23, 2017
  • Revised: October 23, 2017
  • Accepted: February 14, 2018
  • DOI: 10.5812/zjrms.60736

To Cite: Behdad S, Dolatabadi S, Saeidi J. A Simple Method for High-Level Expression of HTLV-1 gp46-I(162-214) Peptide and Its Application for Screening, Zahedan J Res Med Sci. 2018 ; 20(2):e60736. doi: 10.5812/zjrms.60736.

Copyright © 2018, Zahedan Journal of Research in Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited
1. Background
2. Methods
3. Results
4. Discussion
  • 1. Goncalves DU, Proietti FA, Ribas JG, Araujo MG, Pinheiro SR, Guedes AC, et al. Epidemiology, treatment, and prevention of human T-cell leukemia virus type 1-associated diseases. Clin Microbiol Rev. 2010;23(3):577-89. doi: 10.1128/CMR.00063-09. [PubMed: 20610824].
  • 2. Uchenna Tweteise P, Natukunda B, Bazira J. Human T-Cell Lymphotropic Virus Types 1 and 2 Seropositivity among Blood Donors at Mbarara Regional Blood Bank, South Western Uganda. Leuk Res Treatment. 2016;2016:1675326. doi: 10.1155/2016/1675326. [PubMed: 27034840].
  • 3. Santos RF, Conceicao GC, Martins MS, Kraychete A, Penalva MA, Carvalho EM, et al. Prevalence and risk factors for Human T-Lymphotropic Virus Type 1 (HTLV-1) among maintenance hemodialysis patients. BMC Nephrol. 2017;18(1):64. doi: 10.1186/s12882-017-0484-y. [PubMed: 28202003].
  • 4. Abbaszadegan MR, Jafarzadeh N, Sankian M, Varasteh A, Mahmoudi M, Sadeghizadeh M, et al. Truncated MTA-1: a pitfall in ELISA-based immunoassay of HTLV-1 infection. J Biomed Biotechnol. 2008;2008:846371. doi: 10.1155/2008/846371. [PubMed: 18566680].
  • 5. Mosadeghi P, Zarnagh HH, Mohammad-Zadeh M, Salehi Moghaddam M. High-Level Soluble Expression and One-step Purification of HTLV-I P19 Protein in Escherichia coli by Fusion Expression. Iran J Allergy Asthma Immunol. 2015;14(6):624-32. [PubMed: 26725560].
  • 6. Chen R. Bacterial expression systems for recombinant protein production: E. coli and beyond. Biotechnol Adv. 2012;30(5):1102-7. doi: 10.1016/j.biotechadv.2011.09.013. [PubMed: 21968145].
  • 7. Lilley GG, Dolezal O, Hillyard CJ, Bernard C, Hudson PJ. Recombinant single-chain antibody peptide conjugates expressed in Escherichia coli for the rapid diagnosis of HIV. J Immunol Methods. 1994;171(2):211-26. [PubMed: 7515087].
  • 8. Lichty JJ, Malecki JL, Agnew HD, Michelson-Horowitz DJ, Tan S. Comparison of affinity tags for protein purification. Protein Expr Purif. 2005;41(1):98-105. doi: 10.1016/j.pep.2005.01.019. [PubMed: 15802226].
  • 9. Arnau J, Lauritzen C, Petersen GE, Pedersen J. Current strategies for the use of affinity tags and tag removal for the purification of recombinant proteins. Protein Expr Purif. 2006;48(1):1-13. doi: 10.1016/j.pep.2005.12.002. [PubMed: 16427311].
  • 10. Faramarzi R, Dolatabadi S. Expression and purification of recombinant HTLV-I/-II linear epitopes antigen and its application for screening of suspected patients. Iran J Microbiol. 2017;9(1):43-9. [PubMed: 28775823].
  • 11. Hinuma S, Honda S, Tsukamoto K, Sugamura K, Hinuma Y. Production of plasminogen activators by human T-cell leukaemia virus-transformed human T cell lines. Br J Cancer. 1985;51(6):753-9. [PubMed: 2988592].
  • 12. Karimi G, Zadsar M, Pourfathollah AA. Seroprevalence and geographical distribution of human T-lymphotropic virus type 1 among volunteer blood donors in endemic areas of Iran. Virol J. 2017;14(1):14. doi: 10.1186/s12985-017-0693-9. [PubMed: 28137274].
  • 13. Zarnagh HH, Ravanshad M, Pourfatollah AA, Rasaee MJ. Expression and purification of a novel computationally designed antigen for simultaneously detection of htlv-1 and hbv antibodies. Iran J Allergy Asthma Immunol. 2015;14(2):168.
  • 14. Davis GD, Elisee C, Newham DM, Harrison RG. New fusion protein systems designed to give soluble expression in Escherichia coli. Biotechnol Bioeng. 1999;65(4):382-8. [PubMed: 10506413].
  • 15. Hwang PM, Pan JS, Sykes BD. Targeted expression, purification, and cleavage of fusion proteins from inclusion bodies in Escherichia coli. FEBS Lett. 2014;588(2):247-52. doi: 10.1016/j.febslet.2013.09.028. [PubMed: 24076468].
  • 16. Yamaguchi H, Miyazaki M. Refolding techniques for recovering biologically active recombinant proteins from inclusion bodies. Biomolecules. 2014;4(1):235-51. doi: 10.3390/biom4010235. [PubMed: 24970214].
  • 17. Li YM, Li DJ, Xu XJ, Cui M, Zhen HH, Wang Q. Effect of codon optimization on expression levels of human cystatin C in Pichia pastoris. Genet Mol Res. 2014;13(3):4990-5000. doi: 10.4238/2014.July.4.14. [PubMed: 25062487].
  • 18. Young CL, Britton ZT, Robinson AS. Recombinant protein expression and purification: a comprehensive review of affinity tags and microbial applications. Biotechnol J. 2012;7(5):620-34. doi: 10.1002/biot.201100155. [PubMed: 22442034].
  • 19. Mooney JT, Fredericks DP, Christensen T, Bruun Schiodt C, Hearn MT. N-terminal processing of affinity-tagged recombinant proteins purified by IMAC procedures. J Mol Recognit. 2015;28(7):401-12. doi: 10.1002/jmr.2456. [PubMed: 25727088].
  • 20. Kim YE, Hipp MS, Bracher A, Hayer-Hartl M, Hartl FU. Molecular chaperone functions in protein folding and proteostasis. Annu Rev Biochem. 2013;82:323-55. doi: 10.1146/annurev-biochem-060208-092442. [PubMed: 23746257].
  • 21. Xi H, Yuan R, Chen X, Gu T, Cheng Y, Li Z, et al. Purification and on-column refolding of a single-chain antibody fragment against rabies virus glycoprotein expressed in Escherichia coli. Protein Expr Purif. 2016;126:26-32. doi: 10.1016/j.pep.2016.05.004. [PubMed: 27157441].
  • 22. Chen YM, Lee TH, Samuel KP, Okayama A, Tachibana N, Miyoshi I, et al. Antibody reactivity to different regions of human T-cell leukemia virus type 1 gp61 in infected people. J Virol. 1989;63(11):4952-7. [PubMed: 2677406].

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