Zahedan Journal of Research in Medical Sciences

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Ultrasound Effect on Gene Expression of Sex Determining Region Y-box 9 (SOX9) and Transforming Growth Factor β Isoforms in Adipose Stem Cells

Hajar Shafaei 1 , * and Hassan Baghernezhad 2
Authors Information
1 Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, IR Iran
2 Department of Radiology, Sina Hospital, Tabriz University of Medical Sciences, Tabriz, IR Iran
Article information
  • Zahedan Journal of Research in Medical Sciences: April 30, 2016, 18 (4); e6465
  • Published Online: April 27, 2016
  • Article Type: Research Article
  • Received: February 14, 2015
  • Accepted: July 3, 2015
  • DOI: 10.17795/zjrms-6465

To Cite: Shafaei H, Baghernezhad H. Ultrasound Effect on Gene Expression of Sex Determining Region Y-box 9 (SOX9) and Transforming Growth Factor β Isoforms in Adipose Stem Cells, Zahedan J Res Med Sci. 2016 ; 18(4):e6465. doi: 10.17795/zjrms-6465.

Copyright © 2016, Zahedan University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
  • 1. Schugar RC, Robbins PD, Deasy BM. Small molecules in stem cell self-renewal and differentiation. Gene Ther. 2008; 15(2): 126-35[DOI][PubMed]
  • 2. Goldring MB, Tsuchimochi K, Ijiri K. The control of chondrogenesis. J Cell Biochem. 2006; 97(1): 33-44[DOI][PubMed]
  • 3. Hao J, Varshney RR, Wang DA. Engineering osteogenesis and chondrogenesis with gene-enhanced therapeutic cells. Curr Opin Mol Ther. 2009; 11(4): 404-10[PubMed]
  • 4. Huang AH, Farrell MJ, Mauck RL. Mechanics and mechanobiology of mesenchymal stem cell-based engineered cartilage. J Biomech. 2010; 43(1): 128-36[DOI][PubMed]
  • 5. Nowlan NC, Sharpe J, Roddy KA, Prendergast PJ, Murphy P. Mechanobiology of embryonic skeletal development: Insights from animal models. Birth Defects Res C Embryo Today. 2010; 90(3): 203-13[DOI][PubMed]
  • 6. Winter A, Breit S, Parsch D, Benz K, Steck E, Hauner H, et al. Cartilage-like gene expression in differentiated human stem cell spheroids: a comparison of bone marrow-derived and adipose tissue-derived stromal cells. Arthritis Rheum. 2003; 48(2): 418-29[DOI][PubMed]
  • 7. Sekiya I, Vuoristo JT, Larson BL, Prockop DJ. In vitro cartilage formation by human adult stem cells from bone marrow stroma defines the sequence of cellular and molecular events during chondrogenesis. Proc Natl Acad Sci U S A. 2002; 99(7): 4397-402[DOI][PubMed]
  • 8. Mwale F, Girard-Lauriault PL, Wang HT, Lerouge S, Antoniou J, Wertheimer MR. Suppression of genes related to hypertrophy and osteogenesis in committed human mesenchymal stem cells cultured on novel nitrogen-rich plasma polymer coatings. Tissue Eng. 2006; 12(9): 2639-47[DOI][PubMed]
  • 9. Mueller MB, Tuan RS. Functional characterization of hypertrophy in chondrogenesis of human mesenchymal stem cells. Arthritis Rheum. 2008; 58(5): 1377-88[DOI][PubMed]
  • 10. Huang CY, Hagar KL, Frost LE, Sun Y, Cheung HS. Effects of cyclic compressive loading on chondrogenesis of rabbit bone-marrow derived mesenchymal stem cells. Stem Cells. 2004; 22(3): 313-23[DOI][PubMed]
  • 11. Huang CY, Reuben PM, Cheung HS. Temporal expression patterns and corresponding protein inductions of early responsive genes in rabbit bone marrow-derived mesenchymal stem cells under cyclic compressive loading. Stem Cells. 2005; 23(8): 1113-21[DOI][PubMed]
  • 12. Mouw JK, Connelly JT, Wilson CG, Michael KE, Levenston ME. Dynamic compression regulates the expression and synthesis of chondrocyte-specific matrix molecules in bone marrow stromal cells. Stem Cells. 2007; 25(3): 655-63[DOI][PubMed]
  • 13. Cicha I, Goppelt-Struebe M. Connective tissue growth factor: context-dependent functions and mechanisms of regulation. Biofactors. 2009; 35(2): 200-8[DOI][PubMed]
  • 14. Rich JT, Rosova I, Nolta JA, Myckatyn TM, Sandell LJ, McAlinden A. Upregulation of Runx2 and Osterix during in vitro chondrogenesis of human adipose-derived stromal cells. Biochem Biophys Res Commun. 2008; 372(1): 230-5[DOI][PubMed]
  • 15. Klein-Nulend J, Roelofsen J, Sterck JG, Semeins CM, Burger EH. Mechanical loading stimulates the release of transforming growth factor-beta activity by cultured mouse calvariae and periosteal cells. J Cell Physiol. 1995; 163(1): 115-9[DOI][PubMed]
  • 16. Schumann D, Kujat R, Zellner J, Angele MK, Nerlich M, Mayr E, et al. Treatment of human mesenchymal stem cells with pulsed low intensity ultrasound enhances the chondrogenic phenotype in vitro. Biorheology. 2006; 43(3-4): 431-43[PubMed]
  • 17. Knippenberg M, Helder MN, Doulabi BZ, Semeins CM, Wuisman PI, Klein-Nulend J. Adipose tissue-derived mesenchymal stem cells acquire bone cell-like responsiveness to fluid shear stress on osteogenic stimulation. Tissue Eng. 2005; 11(11-12): 1780-8[DOI][PubMed]
  • 18. Korstjens CM, van der Rijt RH, Albers GH, Semeins CM, Klein-Nulend J. Low-intensity pulsed ultrasound affects human articular chondrocytes in vitro. Med Biol Eng Comput. 2008; 46(12): 1263-70[DOI][PubMed]
  • 19. Li J, Wang J, Zou Y, Zhang Y, Long D, Lei L, et al. The influence of delayed compressive stress on TGF-β1-induced chondrogenic differentiation of rat BMSCs through Smad-dependent and Smad-independent pathways. Biomaterials. 2012; 33(33): 8395-405[DOI]
  • 20. Wang SE, Wu FY, Shin I, Qu S, Arteaga CL. Transforming growth factor {beta} (TGF-{beta})-Smad target gene protein tyrosine phosphatase receptor type kappa is required for TGF-{beta} function. Mol Cell Biol. 2005; 25(11): 4703-15[DOI][PubMed]
  • 21. Leucht P, Kim JB, Amasha R, James AW, Girod S, Helms JA. Embryonic origin and Hox status determine progenitor cell fate during adult bone regeneration. Development. 2008; 135(17): 2845-54[DOI][PubMed]
  • 22. Li Z, Kupcsik L, Yao SJ, Alini M, Stoddart MJ. Mechanical load modulates chondrogenesis of human mesenchymal stem cells through the TGF-beta pathway. J Cell Mol Med. 2010; 14(6A): 1338-46[DOI][PubMed]
  • 23. Schumann D, Kujat R, Nerlich M, Angele P. Mechanobiological conditioning of stem cells for cartilage tissue engineering. Biomed Mater Eng. 2006; 16(4 Suppl)-52[PubMed]
  • 24. Cheng K, Xia P, Lin Q, Shen S, Gao M, Ren S, et al. Effects of low-intensity pulsed ultrasound on integrin-FAK-PI3K/Akt mechanochemical transduction in rabbit osteoarthritis chondrocytes. Ultrasound Med Biol. 2014; 40(7): 1609-18[DOI][PubMed]
  • 25. Louw TM, Budhiraja G, Viljoen HJ, Subramanian A. Mechanotransduction of ultrasound is frequency dependent below the cavitation threshold. Ultrasound Med Biol. 2013; 39(7): 1303-19[DOI][PubMed]
  • 26. Yang Z, Sui L, Toh WS, Lee EH, Cao T. Stage-dependent effect of TGF-beta1 on chondrogenic differentiation of human embryonic stem cells. Stem Cells Dev. 2009; 18(6): 929-40[DOI][PubMed]
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