The Cytoprotective Effects of Morus Alba Leaves in Cultured Fetus Fibroblast Cells against Hyperglycemia


Mohammadreza Shams-Ardekani 1 , Abbas Barin 2 , Naiema Vakili-Saatloo 3 , parisa sadighara 3 , *

1 Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Science, Tehran, Iran

2 Department of Clinical Pathology, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran

3 Department of Environmental Health, Food Safety Division, School of Public Health, Tehran University of Medical Science, Tehran, Iran

How to Cite: Shams-Ardekani M, Barin A, Vakili-Saatloo N, sadighara P. The Cytoprotective Effects of Morus Alba Leaves in Cultured Fetus Fibroblast Cells against Hyperglycemia, Zahedan J Res Med Sci. 2013 ; 15(11):e92806.


Zahedan Journal of Research in Medical Sciences: 15 (11); e92806
Published Online: May 28, 2013
Article Type: Research Article
Received: August 19, 2012
Revised: September 20, 2012
Accepted: November 14, 2012




Background : Diabetes is a chronic disease characterized by high blood glucose levels. Medicinal plants have useful source of hypoglycemic compounds and play important role in preventing the progress of this disease. Morus alba leaves is natural therapeutic agent shown hypoglycemic effects. In this study, the protective effects of alcoholic extract Morus alba leaves on fetus fibroblast cells under hyperglycemic condition were assessed.
Materials and Methods : In this experimental study the cells were treated with six different concentrations of extracts. The nontoxic concentration of plant extract was measured with MTT assay in fetus fibroblast cells. Then, the fetus fibroblast cells were incubated with high dose of glucose and plant extract at concentrations of 1000 ppm. DNA synthesis, as marker of cell proliferation was determined.
Results : There were significant changes in cell proliferation in treated group with plant extract. Morus alba leaves could cause cell attachment and proliferation. The attachment of cells to culture plates has been shown to be affected by plant extract.
Conclusion : The data obtained from this study approved that Morus alba leaves has cytoprotective effects against hyperglycemia.


Morus alba Cytoprotective Hyperglycemia

© 2013, Zahedan Journal of Research in Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.


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