The Study of the Effects of Post Treatment with Rosmarinus Officinalis Aqueous Extract the First 48 Hours after Renal Ischemia/Reperfusion in Rats


Saeed Changizi-Ashtiyani 1 , * , Marzieh Zohrabi 2 , Akbar Hassanpoor 3 , Saeed Hajihashemi 1 , Nasser Hosseini 4


1 Kidney and Blood Pressure Research Center, Arak University of Medical Sciences, Arak, Iran

2 Department of Physiology, Arak University of Medical Sciences, Arak, Iran

3 Department of Pathology, Arak University of Medical Sciences, Arak, Iran

4 Department of Medicinal Plants, Arak University Arak, Iran


Zahedan Journal of Research in Medical Sciences: 15 (6); e92930
Published Online: October 29, 2012
Article Type: Research Article
Received: October 26, 2011
Accepted: December 14, 2011




Background: Acute renal failure induced by ischemia / reperfusion (I/R) causes excretory
functional disorders of nephrons. The purpose of this study is to evaluate the intake of oral
rosemary extract (gavage) on hemodynamic changes and tissue damages caused by I/R.
Materials and Methods: This study was conducted on 40 Sprague-Dawley male rats,
composed of 4 groups (n=10) including control, sham, I/R+normal saline (I/R+NS) posttreatment
and I/R+rosemary (I/R+RO) post-treatment. They were fed with 10 ml/kg of 8%
aqueous extract or vehicle as gavages during the first 48 hours after I/R. To induce I/R,
both renal artery and vein were blocked for 30 minutes followed by a reperfusion within
48 hours. Then urine and plasma samples were collected and histological study was also
conducted after removal of both kidneys. Data were analyzed by SPSS-11.5 software. The
one-way analysis of variance (ANOVA) and Duncan test were used for intergroup
comparison and p≤ 0.05 was used as the significant level. The Kruskal–Wallis multiple
comparison test was also used for doing histological analysis
Results: Comparison of renal function between two groups of (I/R+RO) and (I/R+NS),
showed a significant reduction in plasma creatinine (p=0.05), the blood urea nitrogen
(p=0.05), absolute excretion of sodium (p=0.001) and an increase in absolute potassium
excretion (p=0.01). Results of the histological study also indicated a significant decrease in
the vascular congestion, Bowman’s capsule space and oxidative stress in the group
(I/R+RO) in comparison with (I/R+NS).
Conclusion: Treatment with rosemary may be effective in the reduction of the functional
and histological effects of I/R.


Acute renal failure Ischemia/reperfusion Kidney Rat Rosmarinus officinalis

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