Effects of Chronic 5-Bromo-2-Deoxyuridine Administration on Spatial Memory in the Adult Rats

AUTHORS

Mahmoud Hosseini 1 , Abbas Mohammadi pour 2 , * , Azadeh Feizpour 1 , Vahid Ebrahimi 2

1 Department of Physiology, Mashhad University of Medical Sciences, Mashhad, Iran

2 Department of Anatomy, Mashhad University of Medical Sciences, Mashhad, Iran

How to Cite: Hosseini M, Mohammadi pour A, Feizpour A, Ebrahimi V. Effects of Chronic 5-Bromo-2-Deoxyuridine Administration on Spatial Memory in the Adult Rats, Zahedan J Res Med Sci. 2013 ; 15(5):e92977.

ARTICLE INFORMATION

Zahedan Journal of Research in Medical Sciences: 15 (5); e92977
Published Online: November 18, 2012
Article Type: Research Article
Received: February 21, 2012
Accepted: October 22, 2012

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Abstract

Background : 5-Bromo-2-deoxyuridine (BrdU) has been a principal marker for mitotic cells in studies of adult neurogenesis. The method consists of a pulse injection of BrdU into the intraperitoneal cavity followed by a variable survival time allowing for tracking the divided cells and their progeny. However, such exogenous markers may produce toxic effects. Aim of this study was determined the effects of Brdu on spatial memory in the adult rat.
Materials and Methods : 16 Wistar rats were used in this experimental study. The rats were randomly divided into 2 groups (N=8 in each group), as follows: control and Brdu (50 mg/kg). Brdu was administered intraperitoneally for 6 weeks and then animals were used for behavioral testing in the Morris water maze. The data were analyzed with repeated measure’s ANOVA.
Results : Our present findings show that there were no differences in the path length, escape latency and swim speed between control and Brdu-administrated groups.
Conclusion : This study show that Brdu (exogenic proliferation marker) did not has side effects on spatial memory in the adult rats.

Keywords

5-Bromo-2-deoxyuridine Spatial memory Rat

© 2013, Zahedan Journal of Research in Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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