Protective and antioxidant activities of turnip root ethanolic extract against cisplatin induced hepatotoxicity in rats

AUTHORS

Daryoush Mohajeri 1 , * , Yousef Doustar 2 , Ghafour Mousavi 3

AUTHORS INFORMATION

1 Associate Professor of Pathology, Dept. of Pathobiology, School of Veterinary Medicine, Islamic Azad University, Tabriz Branch, Tabriz, Iran.

2 Assistant Professor of Pathology, Dept. of Pathobiology, School of Veterinary Medicine, Islamic Azad University, Tabriz Branch, Iran.

3 Assistant Professor of Veterinary Surgery, Dept. of Clinical Science, School of Veterinary Medicine, Islamic Azad University, Tabriz Branch, Iran.

ARTICLE INFORMATION

Zahedan Journal of Research in Medical Sciences: 13 (9); e93705
Published Online: May 20, 2011
Article Type: Research Article
Received: December 02, 2010
Accepted: December 22, 2010

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Abstract

Background: Cisplatin is an antineoplastic drug and at high doses is hepatotoxic. Oxidative stress has been proven to be involved in cisplatin-induced hepatotoxicity. Because of antioxidant potential of turnip (Brassica rapa. L)  root, the objective of this study was to examine the protective effect of turnip root ethanolic extract (TREE), on cisplatin-induced hepatotoxicity in rat.

Materials and Method: Forty male Wistar rats were randomly allocated into four equal groups. Group 1 was used as control groups 2 and 4 were orally treated with TREE (200 mg/kg) for 15 consecutive days. Groups 3 and 4 received a single intraperitoneal dose of cisplatin (7.5 mg/kg) on the 10th day of the experiment. At the end of experiment, serum levels of aspartate and alanine transaminases, lactate dehydogenase and total bilirubin, albumin and total proteins were assessed. Malondialdehyde, reduced glutathione and activities of superoxide dismutase, catalase and glutathione peroxidase and glutathione reductase were assayed in liver homogenates. Finally, the biochemical findings were matched with histopathological verifications.

Results: In group 4, TREE significantly (p=0.0001) decreased the elevated levels of serum biomarkers of hepatic injury and total bilirubin and significantly increased the reduced levels of serum albumin and total proteins (respectively p=0.001, p=0.032). In this group, TREE significantly (p=0.0001) decreased the lipid peroxidation and elevated the decreased values of hepatic antioxidants. Histopathologically, the changes were in the same direction with biochemical findings.

Conclusion: Because of anti-oxidant potentials of TREE, it may have a protective effect against cisplatin induced hepatotoxicity in rats. [ZJRMS, 2012 13(9): 8-15]

Keywords

Turnip (Brassica rapa. L) root cisplatin hepatotoxicity rat

© 2012, Zahedan Journal of Research in Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
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