Anti-leishmanial effect of nanosilver solutions on Leishmania tropica promastigotes by in-vitro assay

AUTHORS

Ahmad Khosravi 1 , Iraj Sharifi 2 , * , Mohammad Barati 3 , Mahdi Zarean 4 , Marayam Hakimi-Parizi 5

AUTHORS INFORMATION

1 MPH student &DVM, Dermatology & Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran

2 Proffesor of Parasitology, Dermatology & Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran.

3 PhD student of Parasitology, School of Hygiene, Tehran University of Medical Sciences, Tehran, Iran.

4 Instructor of Parasitology, Dermatology & Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran

5 Instructor of Medical Entomology, Dermatology & Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran.

How to Cite: Khosravi A, Sharifi I, Barati M, Zarean M, Hakimi-Parizi M. Anti-leishmanial effect of nanosilver solutions on Leishmania tropica promastigotes by in-vitro assay, Zahedan J Res Med Sci. 2011 ; 13(7):e93813.

ARTICLE INFORMATION

Zahedan Journal of Research in Medical Sciences: 13 (7); e93813
Published Online: May 19, 2011
Article Type: Research Article
Received: July 04, 2011
Accepted: April 10, 2011

Crossmark

CHEKING

READ FULL TEXT
Abstract

Background: At present, nanosilver particles are used against a broad spectrum of microbial agents. The objective of this study was to evaluate the anti-leishmanial effect of nanosilver solutions on L. tropica promastigotes as compared with the trivalent antimony compound by colorimetric assay.

Materials and Method: Promastigots of L. tropica were cultured in RPMI-1640 consisting of 10% fetal calf serum (FCS) and antibiotics at 24±10C. The effect of various concentrations of nanosilver solutions was evaluated in comparison with tartar emetic. Optical density as the result of tetrazolium salts (MTT) reduction by the promastigotes into a formozan product and it was calculated by Elisa reader and IC50 (50% inhibitory concentration), subsequently.

Results: Both, nanosilver particles and tartar emetic inhibited the growth of L. tropica promastigotes. The IC50 for nanosilver solutions was significantly higher (14.9 µg/mL) than the corresponding value (5.3 µg/mL) for the control drug (p<0.001).

Conclusion: Since nanosilver particles showed an effective anti-leishmanial activity, further works are required to evaluate the effect in animal model or human volunteers.

Keywords

Leishmania tropica nanosilver particles colorimetric assay

© 2011, Zahedan Journal of Research in Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

Fulltext

The full text of this article is available on the PDF file.

 

References

  • 1. The References of this article are available on the PDF file.
  • COMMENTS

    LEAVE A COMMENT HERE: