Comparison between C-FOS Expression in Male and Female Mice During Morphine Withdrawal in the Presence and Absence of Acute Administration of Matricaria Recutita

AUTHORS

Mahnaz Kesmati 1 , * , Hamid Galehdari 2 , Azar Massah 3

AUTHORS INFORMATION

1 Associate Prof, Dept of Biology, Faculty of Sciences, Shahid Chamran University, Ahvaz, Iran

2 Associate Prof, Dept of Genetic, Faculty of Sciences, Shahid Chamran University, Ahvaz, Iran

3 Master of Science, Dept of Biology, Faculty of Sciences, Shahid Chamran University, Ahvaz, Iran

How to Cite: Kesmati M, Galehdari H, Massah A. Comparison between C-FOS Expression in Male and Female Mice During Morphine Withdrawal in the Presence and Absence of Acute Administration of Matricaria Recutita, Zahedan J Res Med Sci. 2009 ; 11(2):e94397.

ARTICLE INFORMATION

Zahedan Journal of Research in Medical Sciences: 11 (2); e94397
Published Online: July 15, 2009
Article Type: Research Article
Received: June 02, 2008
Accepted: March 14, 2009

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Abstract

Background: There are some evidences that indicate there are sexual differences in drug abuse
and response to synthetic and herbal drugs. It has been shown that the expression of C-FOS
increases in many areas of brain during morphine withdrawal. Concerning the sedative effect of
Matricaria recutita extract, the aim of this study was to compare expression of C-FOS transcription
factor during morphine withdrawal with and without acute administration of Matricaria recutita on
male and female adult mice.
Materials and Methods: This study was done at Shahid Chamran University of Ahvaz in 2007 on
NMRI mice. Male and female mice were assigned into 8 groups (morphine + saline; morphine +
naloxone; morphine + Matricaria recutita + naloxone; and morphine + saline + naloxone). To
develop morphine dependency, increasing doses of morphine (20, 40, 80 mg/kg injected
subcutaneously) for 4 days. Mice received a final morphine injection (40 mg/kg) 3hours prior to
naloxone (5 mg/kg) on the day of testing (day 4). Matricaria recutita extract whit a dose of 30
mg/kg was administered intraperitoneally 5 minutes before naloxone injection. In cellular study,
90minute after naloxone injection, mice were decapitated and their brains were separated, then
mRNA was extracted from brain tissue. Using DIG-labeled DNA probe of C-FOS, beta-actin and
dot blot technique, expression of C-FOS was analyzed by Zero Dscan software. Statistical
evaluation of data was performed using student t-test and ANOVA with one factor followed by
Duncan test in SPSS software. P values less than 0.05 were considered significant.
Results: The rate of expression of C-FOS increased in male mice but decreased significantly in
female mice after naloxone-precipitated abstinence) P<0.01) . Matricaria recutita attenuated the
rate of expression of C-FOS in male mice but it showed synergistic effect on it in female mice
( P<0.05) .
Conclusion: It seems that the cellular processes involving morphine dependency and response to
treatment with Matricaria recutita are sex dependent and are affected by sexual hormones.

Keywords

Morphine dependency C-fos Matricaria recutita Mice

© 2009, Zahedan Journal of Research in Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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