Effect of subcutaneous injection of morphine on activity of neurons in the nucleus cuneiformis of rat

AUTHORS

Abbas Haghparast 1 , * , AmirMohammad Alizadeh 1 , Fereshteh Motamedi 1

AUTHORS INFORMATION

1 Neuroscience Research Center, Shahid Beheshti University of Medical Sciences and Health Services, Tehran, Iran.

ARTICLE INFORMATION

Zahedan Journal of Research in Medical Sciences: 10 (4); e94508
Published Online: December 17, 2008
Article Type: Research Article
Received: February 28, 2008
Accepted: November 22, 2008

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Abstract

Background: The similarities between periaqueductal gray matter and the nucleus cuneiformis in
both ultrastructural and functional levels suggest that this nucleus may play an important role in
the morphine-induced analgesia. This study was designed to determine neuronal activity and
responsiveness to peripheral morphine administration in the nucleus coneiformis of rat.
Materials and Methods: In this study, neural activity of cuneiform neurons in response to
peripheral administration of morphine was recorded by extracellular single unit recording
technique. Firing rate of neurons was recorded in four groups: intact group (n=19) to determine
the spontaneous (baseline) activity, saline group (n=20), morphine group (n=39) and morphine +
naloxone group (n=12), before and after drug administration.
Results: Our findings showed that the firing rate in majority of cuneiform neurons decreased
after morphine (3.8 mg/kg; SC) administration. Activity of neurons (n=39) in the cuneiform neurons
was reduced significantly (P<0.01) after morphine injection (6.66±0.67 spike/sec) in comparison
with pre-injection time (12.47±1.84 spike/sec) and the saline group (11.6±1.58 spike/sec). The
firing rate and response pattern of many of neurons in response to peripheral application of
morphine were reversed after naloxone injection (2 mg/kg; SC) in this nucleus.
Conclusion: Based on the above findings, we suggest that the changes of activity pattern in
spontaneous activity of cuneiform neurons in response to peripheral administration of morphine
maybe resulted from direct action of morphine on opioid receptors in the nucleus cuneiformis.
Nevertheless, the role of pain transmission and modulation pathways are still important in the
antinociceptive effect of morphine as well.

Keywords

Nucleus cuneiformis Morphine Neural activity Single unit recording Pain Rat

© 2008, Zahedan Journal of Research in Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
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