Zahedan Journal of Research in Medical Sciences

Published by: Kowsar

The Sensitivity of the PCR Method for Detection of Coxiella burnetii in the Milk Samples

Mohammad Kargar 1 , * , Afsaneh Rashidi 1 , Abbass Doosti 2 , Akram Najafi 3 and Sadegh Ghorbani-Dalini 4
Authors Information
1 Department of Microbiology, Jahrom Branch, Islamic Azad University, Jahrom, IR Iran
2 Biotechnology Research Center, Shahrekord Branch, Islamic Azad University, Shahrekord, IR Iran
3 Persian Gulf Marine Biotechnology Medicine Research Center, Bushehr University of Medical Sciences, Bushehr, IR Iran
4 Department of Microbiology, Jahrom Branch, Young Researcher’s Club, Islamic Azad University, Jahrom, IR Iran
Article information
  • Zahedan Journal of Research in Medical Sciences: June 28, 2015, 17 (6)
  • Published Online: June 27, 2015
  • Article Type: Research Article
  • Received: January 13, 2014
  • Accepted: April 11, 2014
  • DOI: 10.17795/zjrms988

To Cite: Kargar M, Rashidi A, Doosti A, Najafi A, Ghorbani-Dalini S. The Sensitivity of the PCR Method for Detection of Coxiella burnetii in the Milk Samples, Zahedan J Res Med Sci. 2015 ; 17(6):-. doi: 10.17795/zjrms988.

Copyright © 2015, Zahedan University of Medical Sciences.This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
  • 1. Parker NR, Barralet JH, Bell AM. Q fever. Lancet. 2006; 367(9511): 679-88[DOI][PubMed]
  • 2. Fretz R, Schaeren W, Tanner M, Baumgartner A. Screening of various foodstuffs for occurrence of Coxiella burnetii in Switzerland. Int J Food Microbiol. 2007; 116(3): 414-8[DOI][PubMed]
  • 3. Psaroulaki A, Hadjichristodoulou C, Loukaides F, Soteriades E, Konstantinidis A, Papastergiou P, et al. Epidemiological study of Q fever in humans, ruminant animals, and ticks in Cyprus using a geographical information system. Eur J Clin Microbiol Infect Dis. 2006; 25(9): 576-86[DOI][PubMed]
  • 4. Angelakis E, Raoult D. Q Fever. Vet Microbiol. 2010; 140(3-4): 297-309[DOI][PubMed]
  • 5. Kersh GJ, Wolfe TM, Fitzpatrick KA, Candee AJ, Oliver LD, Patterson NE, et al. Presence of Coxiella burnetii DNA in the environment of the United States, 2006 to 2008. Appl Environ Microbiol. 2010; 76(13): 4469-75[DOI][PubMed]
  • 6. Roest HI, Tilburg JJ, van der Hoek W, Vellema P, van Zijderveld FG, Klaassen CH, et al. The Q fever epidemic in The Netherlands: history, onset, response and reflection. Epidemiol Infect. 2011; 139(1): 1-12[DOI][PubMed]
  • 7. Vicari N, Faccini S, Ricchi M, Garbarino C, Decastelli L, Boldini M, et al. Occurrence of Coxiella burnetii in bulk tank milk from northwestern Italy. Vet Rec. 2013; 172(26): 687[DOI][PubMed]
  • 8. Kim SG, Kim EH, Lafferty CJ, Dubovi E. Coxiella burnetii in bulk tank milk samples, United States. Emerg Infect Dis. 2005; 11(4): 619-21[DOI][PubMed]
  • 9. Berri M, Laroucau K, Rodolakis A. The detection of Coxiella burnetii from ovine genital swabs, milk and fecal samples by the use of a single touchdown polymerase chain reaction. Vet Microbiol. 2000; 72(3-4): 285-93[PubMed]
  • 10. Manual of diagnostic tests and vaccines for terrestrial animals 2013. 2012;
  • 11. Rahimi E, Doosti A, Ameri M, Kabiri E, Sharifian B. Detection of Coxiella burnetii by nested PCR in bulk milk samples from dairy bovine, ovine, and caprine herds in Iran. Zoonoses Public Health. 2010; 57(7-8): 38-41[PubMed]
  • 12. Berri M, Arricau-Bouvery N, Rodolakis A. PCR detection of microbial pathogens (Methods in Molecular Biology). 2002; : 153-61[DOI]
  • 13. Hoover TA, Vodkin MH, Williams JC. A Coxiella burnetti repeated DNA element resembling a bacterial insertion sequence. J Bacteriol. 1992; 174(17): 5540-8[PubMed]
  • 14. Zhang GQ, Nguyen SV, To H, Ogawa M, Hotta A, Yamaguchi T, et al. Clinical evaluation of a new PCR assay for detection of Coxiella burnetii in human serum samples. J Clin Microbiol. 1998; 36(1): 77-80[PubMed]
  • 15. Loftis AD, Priestley RA, Massung RF. Detection of Coxiella burnetii in commercially available raw milk from the United States. Foodborne Pathog Dis. 2010; 7(12): 1453-6[DOI][PubMed]
  • 16. Klee SR, Tyczka J, Ellerbrok H, Franz T, Linke S, Baljer G, et al. Highly sensitive real-time PCR for specific detection and quantification of Coxiella burnetii. BMC Microbiol. 2006; 6: 2[DOI][PubMed]
  • 17. Vaidya VM, Malik SV, Kaur S, Kumar S, Barbuddhe SB. Comparison of PCR, immunofluorescence assay, and pathogen isolation for diagnosis of q fever in humans with spontaneous abortions. J Clin Microbiol. 2008; 46(6): 2038-44[DOI][PubMed]
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:



Create Citiation Alert
via Google Reader

Readers' Comments