The effect of hepatocyte growth factor on mouse oocyte in vitro maturation and subsequent fertilization and embryo development

AUTHORS

Mohammad Hadi Bahadori 1 , mahnaz azarnia 2 , Fatemeh Ghasemian 3 , *

1 Associate Professor of Anatomy, Cellular and Molecular Research Center, Guilan University of Medical Sciences and Health Services, Rasht, Iran

2 Associate Professor of Biology, School of Science, Kharazmi (Tarbiat moallem) University of Tehran, Iran.

3 MSc of Biology, School of Science, Kharazmi (Tarbiat moallem) University of Tehran, Iran

How to Cite: Bahadori M H, azarnia M, Ghasemian F. The effect of hepatocyte growth factor on mouse oocyte in vitro maturation and subsequent fertilization and embryo development, Zahedan J Res Med Sci. 2011 ; 13(2):e94062.

ARTICLE INFORMATION

Zahedan Journal of Research in Medical Sciences: 13 (2); e94062
Published Online: September 06, 2010
Article Type: Research Article
Received: May 13, 2010
Accepted: June 15, 2010

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Abstract

Background: Oocyte invitro maturation is an enormously promising technology for the treatment of infertility, yet its clinical application remains limited owing to poor success rates. Therefore, this study was devised to evaluate the effect of hepatocyte growth factor (HGF) on in vitro maturation of immature mouse oocytes and resulting embryos development.   
Materials and Method: Cumulus – oocyte complex and germinal vesicle were obtained from eighteen 6-8 weeks-old female NMRI mice 46-48 hours after administration of an injection of 5 IU PMSG (Pregnant Mares’ Serum Gonadotrophin). Oocytes were culture in TCM199 (Tissue culture medium-199) supplemented with dosages of 0, 10, 20, 50 and 100 ng/ml of HGF. After 24 hours, metaphase ІІ oocytes were co-incubated with sperms for 4-6 hours in T6 medium. Following isolation of two pronucleus embryos, cleavage of embryos was assessed in the same medium till blastocyst stage. The number of oocytes and embryos was recorded under an invert microscope and the rate of oocyte maturation, fertilization and embryos cleavage until blastocyst stage compared using of student χ2 test.
Results: In all compared groups, oocytes growth and embryos development rate in the 20 ng/ml of HGF treatment group was significantly higher (p<0.05) than the control group (p<0.05).
Conclusion: 20 ng/ml of HGF improved the nuclear maturation and embryo development up to blastocyst stage during culture condition.

Keywords

Hepatocyte growth factor IVM IVF embryonic development

© 2011, Zahedan Journal of Research in Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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