Zahedan Journal of Research in Medical Sciences

Published by: Kowsar

The Sea Cucumber Body Wall Extract Promoted in Vitro Maturation of NMRI Mice Follicles at Germinal Vesicle Stage

Mitra Khalilzadeh 1 , Javad Baharara 1 , * , Mohsen Jalali 2 , Farideh Namvar 3 and Elaheh Amini 4
Authors Information
1 Department of Biology, Faculty of Sciences, Mashhad Branch, Islamic Azad University, Mashhad, IR Iran
2 Montaserieh Infertility Treatment Center, Mashhad University of Medical Sciences, Mashhad, IR Iran
3 Institute of Tropical Forestry and Forest Products, University Putra Malaysia, Selangor, Malaysia
4 Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, IR Iran
Article information
  • Zahedan Journal of Research in Medical Sciences: August 2016, 18 (8); e3021
  • Published Online: July 31, 2016
  • Article Type: Research Article
  • Received: June 16, 2015
  • Revised: July 16, 2015
  • Accepted: July 19, 2016
  • DOI: 10.17795/zjrms-3021

To Cite: Khalilzadeh M, Baharara J, Jalali M, Namvar F, Amini E. The Sea Cucumber Body Wall Extract Promoted in Vitro Maturation of NMRI Mice Follicles at Germinal Vesicle Stage, Zahedan J Res Med Sci. 2016 ; 18(8):e3021. doi: 10.17795/zjrms-3021.

Copyright © 2016, Zahedan University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Methods
4. Results
5. Discussion
  • 1. Tao P, Coates R, Maycock B. Investigating marital relationship in infertility: a systematic review of quantitative studies. J Reprod Infertil. 2012; 13(2): 71-80[PubMed]
  • 2. Reichman DE, Politch J, Ginsburg ES, Racowsky C. Extended in vitro maturation of immature oocytes from stimulated cycles: an analysis of fertilization potential, embryo development, and reproductive outcomes. J Assist Reprod Genet. 2010; 27(7): 347-56[DOI][PubMed]
  • 3. Farsi MM, Kamali N, Pourghasem M. Embryological aspects of oocyte in vitro maturation. Int J Mol Cell Med. 2013; 2(3): 99-109[PubMed]
  • 4. Farsi MM, Jorsaraei SG, Esmaelzadeh S, Golaipour MJ. In vitro maturation of germinal vesicle oocytes in stimulated intracytoplasmic sperm injection cycles. Cell J. 2011; 13(2): 73-8[PubMed]
  • 5. Rajabi-Toustani R, Motamedi-Mojdehi R, Roostaei A, Motamedi-Mojdehi R. Effect of papaver rhoeas L. extract on in vitro maturation of sheep oocytes. Small Rumin Res. 2013; 114(1): 146-51
  • 6. Kobayashi M, Lee ES, Fukui Y. Cysteamine or beta-mercaptoethanol added to a defined maturation medium improves blastocyst formation of porcine oocytes after intracytoplasmic sperm injection. Theriogenology. 2006; 65(6): 1191-9[DOI][PubMed]
  • 7. You J, Kim J, Lim J, Lee E. Anthocyanin stimulates in vitro development of cloned pig embryos by increasing the intracellular glutathione level and inhibiting reactive oxygen species. Theriogenology. 2010; 74(5): 777-85[DOI][PubMed]
  • 8. Al-maeeni IM, Samaree KW, Abdulrahman LY. Study the effect of addition alcoholic extract of Tribulus terrestris on bovine oocyte maturation in vitro ( IVM ). Iraqi J Vet Med. 2012; 36(2): 199-203
  • 9. Inoue T, Ono Y, Yonezawa Y, Oi M, Kobayashi N, Kishi J. Oocyte quality improvement using a herbal medicine comprising 7 crude drugs. Open J Obstetrics Gynecol. 2013; : 195-202
  • 10. Ushiroyama T, Yokoyama N, Hakukawa M, Sakuma K, Ichikawa F, Yoshida S. Clinical efficacy of macrophage-activating Chinese mixed herbs (MACH) in improvement of embryo qualities in women with long-term infertility of unknown etiology. Am J Chin Med. 2012; 40(1): 1-10[DOI][PubMed]
  • 11. Jha RK, Zi-rong X. Biomedical compounds from marine organisms. Mar Drugs. 2004; 2(3): 123-46
  • 12. Mamelona J, Pelletier E, Girard-Lalancette K. Quantification of phenolic contents and antioxidant capacity of Atlantic sea cucumber, Cucumaria frondosa. Food Chem. 2007; 104(3): 1040-7
  • 13. Althunibat OY, Ridzwan BH, Taher M, Daud JM, Jauhari Arief Ichwan S, Qaralleh H. Antioxidant and cytotoxic properties of two sea cucumbers, Holothuria edulis lesson and Stichopus horrens Selenka. Acta Biol Hung. 2013; 64(1): 10-20[DOI][PubMed]
  • 14. Osama YA, Ridzwan BH, Muhammad T, Jamaludin MD, Masa-Aki I. In Vitro Antioxidant and Antiproliferative Activities of ThreeMalaysian Sea Cucumber Species. European J Sci Res. 2009; 37(3): 376-87
  • 15. Zhang X, Gao F. Natural antioxidants for health promotion and disease prevention. Ethnopharmacol. 2014; 266(5): 679-86
  • 16. Rajabi-Toustani R, Motamedi-Mojdehi R, Roostaei-Ali Mehr M, Motamedi-Mojdehi R. Effect of Papaver rhoeas L. extract on in vitro maturation of sheep oocytes. Small Ruminant Res. 2013; 114(1): 146-51
  • 17. Baharara J, Amini E, Kerachian MA, Soltani M. The osteogenic differentiation stimulating activity of Sea cucumber methanolic crude extraction on rat bone marrow mesenchymal stem cells. Iran J Basic Med Sci. 2014; 17(8): 626-31[PubMed]
  • 18. Hara S, Takahashi T, Amita M, Matsuo K, Igarashi H, Kurachi H. Pioglitazone counteracts the tumor necrosis factor-alpha inhibition of follicle-stimulating hormone-induced follicular development and estradiol production in an in vitro mouse preantral follicle culture system. J Ovarian Res. 2013; 6(1): 69[DOI][PubMed]
  • 19. Adriaenssens T, Mazoyer C, Segers I, Wathlet S, Smitz J. Differences in collagen expression in cumulus cells after exposure to highly purified menotropin or recombinant follicle-stimulating hormone in a mouse follicle culture model. Biol Reprod. 2009; 80(5): 1015-25[DOI][PubMed]
  • 20. Soltani M, Baharara J. Antioxidant and antiprolifereative capacity of dichloromethane extract of holoturia leucospilota sea cucumber. Int J Mol Cell Med. 2014; 20(14): 1-9
  • 21. Abdo M, Hisheh S, Arfuso F, Dharmarajan A. The expression of tumor necrosis factor-alpha, its receptors and steroidogenic acute regulatory protein during corpus luteum regression. Reprod Biol Endocrinol. 2008; 6: 50[DOI][PubMed]
  • 22. Nabiuni M, Kakai M, Nazari Z, Karimzadeh L. Effect of honey bee venom on in vitro maturation of preantral follicles in NMRI mice [ in persian]. J Sci Kharazmi Uni. 2013; 13(1): 749-60
  • 23. Barakat IAH, Hassan AM, Alam SS, Khalil WKB. Genetic and biochemical effects of natural extracts on in Vitro maturation of Egyptian buffalo oocytes. Cytologia. 2010; 75(3): 243-53
  • 24. Althunibat OY, Hashim RB, Taher M, Daud JM, Aki M. In Vitro Antioxidant and Antiproliferative Activities of Three Malaysian Sea Cucumber Species. Eur J Sci Res. 2009; 37(3): 376-87
  • 25. Esmat AY, Said MM, Soliman AA, El-Masry KS, Badiea EA. Bioactive compounds, antioxidant potential, and hepatoprotective activity of sea cucumber (Holothuria atra) against thioacetamide intoxication in rats. Nutrition. 2013; 29(1): 258-67[DOI][PubMed]
  • 26. Tavana S, Eimani H, Azarnia M, Shahverdi A, Eftekhari-Yazdi P. Effects of Saffron (Crocus sativus L.) Aqueous Extract on In vitro Maturation, Fertilization and Embryo Development of Mouse Oocytes. Cell J. 2012; 13(4): 259-64[PubMed]
  • 27. Wang ZG, Yu SD, Xu ZR. Improvement in bovine embryo production in vitro by treatment with green tea polyphenols during in vitro maturation of oocytes. Anim Reprod Sci. 2007; 100(1-2): 22-31[DOI][PubMed]
  • 28. Golkar-Narenji A, Eimani H, Samadi F. Effect of Papaver rhoeas extract on in vitro maturation and developmental competence of immature mouse oocytes. Reprod Med Biol. 2010; 9: 211-5
  • 29. Du G, Li M, Ma F, Liang D. Antioxidant capacity and the relationship with polyphenol and vitamin C in Actinidia fruits. Food Chem. 2009; 113: 557-62
  • 30. Hara S, Takahashi T, Amita M, Igarashi H, Tsutsumi S, Kurachi H. Bezafibrate restores the inhibition of FSH-induced follicular development and steroidogenesis by tumor necrosis factor-alpha through peroxisome proliferator-activated receptor-gamma pathway in an in vitro mouse preantral follicle culture. Biol Reprod. 2011; 85(5): 895-906[DOI][PubMed]
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