Zahedan Journal of Research in Medical Sciences

Research Article

31/7/2016

The Sea Cucumber Body Wall Extract Promoted in Vitro Maturation of NMRI Mice Follicles at Germinal Vesicle Stage

Mitra Khalilzadeh, Javad Baharara, Mohsen Jalali, Farideh Namvar, Elaheh Amini

Methods: In this experimental study, Preantral follicles at germinal vesicle stage were collected from 24 - 26 days NMRI female mouse ovaries and transferred to IVM medium supplemented with different concentration of sea cucumber methanol extract (5, 10, 20, 30 µg/mL). Antioxidant capacity of sea cucumber extract was evaluated using DPPH (2, 2-diphenyl-1-picrylhydrazyl) assay. Further, oocyte maturation and TNF-α expression were recorded till day 10^th. Data were analyzed by one-way analysis of variance (ANOVA) through SPSS 16.

Results: The percentage of arrested oocyte at germinal vesicle stage in the control group was significantly (P < 0.05) higher than treated group (20 µg/mL). There were significant (P < 0.01) differences in percentage of maturated oocyte to metaphase II (MII) stage between treated and control group. DPPH radical scavenging capacity and reduction of TNF-α expression in treated oocyte demonstrated that sea cucumber increased rate of oocyte maturation. In addition, the treated oocyte (20 µg/mL) achieved the highest percentage of MII stage.

Research Article

31/7/2016

Appropriate Cutoff Points for Body Mass Index and Waist-To-Hip Ratio for a Large Sample of Adult in Bandar Abbas, Iran

Alireza Shahab Jahanlou, Kamiar Kouzekanani

Methods: The study was cross-sectional. The data were obtained from 13,691 females and 5,675 males between 2009 and 2014. Body composition indices were measured by bioelectrical impedance method (BIA). Percentage of body fat (PBF), total fat (TF), BMI, and WHR were determined. Data analysis included t-test, chi-square test, receiver-operating characteristic (ROC) curves, Fisher’s r-to-z transformation, linear regression, and Hanely formula.

Results: In the WHR classification, nearly 78% of obese subjects had been wrongly classified as non-obese, but only 15.8% of obese females were wrongly classified as non-obese. The WHR was ineffective in detecting obesity among males between 20 and 39 years old. By the BMI classification, 42.5% of obese males had been wrongly classified as non-obese, but it was effective in detecting obesity in females between the ages of 20 and 39 years.

Research Article

31/7/2016

Cloning and Sequencing of Truncated Toxoplasma gondii Subtilisin-Like 1 Antigen

Ahmad Rouhizadeh, Ata A Ghadiri, Mohammad Razi Jalali, Masoud Ghorbanpour, Mohammad Hossein Razi Jalali

Background: Toxoplasma gondii is an obligate intracellular protozoan parasite which has significant medical and veterinary impact on all around the world. Tracking of specific antigens or antibodies for toxoplasmosis is the main choice in its diagnosis. Recombinant proteins will improve sensitivity and specificity and reduce problems of standardization and reproducibility of diagnostic kits. Toxoplasma gondii Subtilisin-like protein (TgSUB1) is a novel example of a glycosylphosphatidylinositol) GPI (-anchored protein which can be considered as a potential marker for serodiagnosis of toxoplasmosis.

Methods: In this experimental study, using bioinformatics softwares Parker Hydrophilicity prediction and Bepipred linear Epitope prediction to select best highly antigenic region of this protein, a 744 bp fragment was amplified by polymerase chain reaction (PCR) on cDNA obtained from T. gondii RNA. The PCR product was cloned in PCR2.1 vector and subcloned into expression pET28a vector. The PCR2.1-SUB1 and PET28a-SUB1constructs were analyzed by PCR, restriction analysis and sequencing.

Research Article

31/7/2016

Assessment of Electrolyte Free Water Clearance in Renal Transplant Recipients

Abbasali Zeraati, Maryam Hami, Farzaneh Sharifipour, Mohammad Reza Reyahi

Methods: A cross-sectional study was conducted to determine three-hour creatinine clearance (CCl), E-CH₂O, and percent urine output in 3 hours after administering 20 mL/Kg oral water loading following 12-hour fasting, in 22 renal transplant recipients with good graft function (creatinine < 1.5 mg/dL) and 26 healthy controls. Patients with diabetes mellitus, hypertension, ischemic heart disease, or liver disease, hypovolemia and hypoalbuminemia were excluded. Statistical analysis was done using t-test, K-square, and Pearson’s correlation test.

Results: We recruited 25 recipients (73% patients in living and 27% in cadaveric donor transplantation) and 26 healthy control groups.3 patients refused to collect urine and finally 22 patients finished the protocol. The mean age of subjects was 37.68 ± 13.88 and 31.40 ± 8.20 years old in renal transplant recipients and control groups, respectively. In patient group male to female ratio was 9/13 and in control group was14/12. Although the 3h-CCl was similar (126.49 ± 53.52 vs. 109.99 ± 47.06 mL/min) in two groups (P > 0.05), the E-CH2O (1.83 ± 1.22 vs. 2.94 ± 2.02) and 3-hour urine output percent (55.93 ± 22.86 vs74.11 ± 30.38) in kidney recipients was significantly lower than healthy controls (P < 0.05). There wasn’t any significant correlation between E-CH2O and 3h-CCl in renal transplant recipients. Donor source and gender did not affect 3h-CCl and E-CH2O in renal transplant patients.

Research Article

1/8/2016

A Gene Expression Profile of Alzheimer’s Disease Using Microarray Technology

Marie Barati, Mansour Ebrahim

Background: Alzheimer’s disease is a form of dementia in old age. Alzheimer’s disease is an incurable disease, usually shows its symptoms in the seventh decade of human life. This disease can exist in an individual for years without visible symptoms. The causes of this disease is still unknown although many studies have been done on this disease. Alzheimer’s disease appears in an old individual based on genetic and accidental factors.

Research Article

31/7/2016

Comparison of the Effects of Acetaminophen Plus Ibuprofen to Treat Fever Than any of the Two Alone in Febrile Children

Noor Mohammad Noori, Ghasem Miri-Aliabad, Tahereh Boryri, Alireza Teimouri, Gholamreza Soleimani

Methods: This Double-blind clinical trial study was done on 540 children with 38°C to 41°C as body temperature. Eligible children after considering inclusion criteria divided in three groups randomly. First group of 183 patients administrated with acetaminophen, the second and the third groups of 178 and 179 patients with ibuprofen and combination. The first dose of antipyretic drug was administered to the patient under the supervision of a physician or nurse. After explanation of benefits and marginal effects to the parents if they accepted the conditions their children were admitted to the study. Parents were free if they wish to withdraw the study before completing. Information of each patient was recorded on a form. The data were analyzed by descriptive statistic, one-way ANOVA and SPSS software version 16.

Results: Out of sample 60.6% were boy. The mean age of children treated with acetaminophen, ibuprofen and combination therapy was 2.21 ± 2.49, 3.00 ± 2.92 and 2.22 ± 2.33 years in the order given. The results showed statistical difference in two (F = 4.45 and P = 0.012) and four hours (F = 3.11 and P = 0.045) after taking drug. A significant difference not observed in the value of temperature decrease within 2 - 4 hours after drug intake, (F = 2.49, P=0.084) but in the time of 0-2 (P = 0.012) and 4-6 hours (P = 0.001) was observed.

Research Article

31/7/2016

Possible Involvement of Beta-Adrenergic Receptors on Nociceptin/Orphanin FQ Induced Food Consumption in Male Rats

Mohammad Reza Hajinezhad, Bahram Shohreh

Methods: In this experimental study were used male rats (250-300 g). In first two experiments, rats received intraperitoneal injections of isoproterenol (0, 0.5, 1 and 2 mg/kg) and Nociceptin agonist 64 - 6198 (0, 1, 2 and 4 mg/kg) respectively. In third experiment 64 - 6198 (4 mg/kg) and (2 mg/kg) isoproterenol were injected. In experiments 4 effects of isoproterenol (0.5 mg/kg) and Nociceptin antagonist (8 mg/kg) on food consumption was investigated. Latency time to feeding was examined in experiment 5. The data were statistically analyzed using One-way ANOVA and Tukey test.

Results: Latency time to feeding increased and decreased by isoproterenol and nociceptin/orphanin, respectively. Administration of N/OFQ increased cumulative food intake in rats (P < 0.05). The i.p. injection of isoproterenol decreased food intake and also the hyperphagic effect of N/OFQ agonist is inhibited by isoproterenol, whereas sub-anorectic doses of isoproterenol and N/OFQ antagonist synergistically attenuated cumulative feed consumption when combined (P < 0.01).

Short Communication

31/7/2016

Comparison of Rate and Progression of Myopia Among Carpet Weaver and Non-Weaver Members of the Weaver's Families in Birjand

Mohammad Hossien Davari, Hoda Gheitasi, Esmat Davari

Short Communication

31/7/2016

Relationship Between Serum Ferritin, TIBC Level and Helicobacter pylori Infection

Kaveh Tari, Zahra Shamsi, Arezou Rahimi, Amir Atashi

Short Communication

31/7/2016

Protective Effects of Aloe Vera on Superovulated Oocytes and Folliculogenesis in Diabetic Mice

Mahsa Afrough, Naeem Erfani Majd, Hossein Najafzadeh

Zahedan Journal of Research in Medical Sciences: Aug 2016, 18 (8), 10 articles.